中文摘要：

目的探讨CCAAT/增强子结合蛋白β（C/EBPβ）基因对HeLa细胞增殖、凋亡和定向迁移的影响。方法用噻唑蓝法检测C/EBPβ基因重组质粒转染组、空质粒转染组和未转染组对HeLa细胞增殖影响;用annexinV/PI双色法流式细胞仪检测HeLa细胞凋亡;Transwell侵袭转移模型评价HeLa细胞迁移情况,其中,不同上室中分别加入C/EBPβ基因重组质粒转染组、空质粒转染组和未转染组的HeLa细胞,下室中加入含20%胎牛血清（FBS）培养基。结果转染组、空质粒组和未转染组HeLa细胞吸光度（A）值分别为（0.38±0.12）、（0.59±0.17）和（0.68±0.17）,组间比较,差异有统计学意义（F=9.769,P〈0.01）;转染组、空质粒组和未转染组HeLa细胞凋亡率分别为（6.99±0.75）%、（3.48±0.74）%和（3.23±1.07）%,转染组与空质粒组和未转染组比较,差异有统计学意义（F=17.541,P〈0.01）;与空质粒组和未转染组比较,转染组细胞迁移能力减弱,细胞穿膜数分别为（16.0±2.3）、（39.0±3.2）和（39.6±2.6）个,组间比较,差异有统计学意义（F=121.785,P〈0.01）。结论 C/EBPβ基因能抑制子宫颈癌HeLa细胞增殖和迁移能力,并促进细胞凋亡。

英文摘要：

Objective To explore the effect of CCAAT/enhancer binding protein β （C/EBPβ） gene on prolifera- tion, apoptosis, and migration of HeLa cells. Methods The HeLa cells were cultured and assigned into C/EBPβ vector pcDNA3.1 transfection group, empty vector pcDNA3.1 transfection group, and non-transfection group. The proliferation of the HeLa cells was determined with 3- （ 4,5-dimethylthiazole-2-yl ） -2,5-diphenyl tetrazolium bromide （MTT） assay. The apoptosis of the HeLa cells was detected with annexin V/PI double staining flow cytometry assay. The migration of HeLa cells was measured with Transwell assay, in which the upper chambers containing C/EBP~ vector transfection, empty transfection,and non-transfection HeLa cells and the lower chambers containing 20% phosphate buffer solution culture medium. Results The absorbance value of C/EBPβ gene vector transfection, empty vector transfection, and non- transfection HeLa cells group 0. 38 ± 0. 12,0. 59±0. 17, and 0. 68 ± 0. 17, respectively, with significant differences among the groups（P 〈 0. 01 ）. The apoptosis rate of C/EBPI3 gene vector transfection, empty vector transfection, and non-trans- fection HeLa cells were 6. 99 ± 0. 75,3.48 ± 0. 74, and 3.23 ± 1.07, respectively, with significant differences among the three groups（P 〈 0. 01 ）. The number of C/EBPβ gene vector transfection HeLa cells penetrating through the membrane were significantly reduced compared with those empty vector transfection and non-transfection HeLa cells （16 ± 2. 3 vs 39± 3.2 and 39. 6 ± 2. 6）. Conclusion C/EBP β gene can inhibit proliferation and migration of HeLa cells and promote the apoptosis of the cells.