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贵州省甘蓝型油菜主推品种的SSR遗传多样性分析
  • ISSN号:1001-3881
  • 期刊名称:《机床与液压》
  • 时间:0
  • 分类:S[农业科学]
  • 作者机构:[1]贵州省油菜研究所,贵州贵阳550008
  • 相关基金:贵州省农委育种专项[黔农育专字(2012)026号];贵州省科技厅农业攻关计划项目,中央支持地方平台建设项目[黔科条中补地(2011)4001];贵州省科技厅农业攻关项目[黔科合 NY 字(2011)3088号].
中文摘要:

[目的]从分子水平上了解贵州省甘蓝型油菜主推品种的遗传多样性。[方法]利用国家油菜区域试验 DNA指纹鉴定使用的40对 SSR引物对贵州省9个甘蓝型油菜主推品种进行多态性检测,并进行聚类分析。[结果]试验共扩增出条带191个,其中多态性条带143个,多态性比率为75%。经聚类分析,9个油菜品种被分为 A、B2簇,其中 A簇包含7个品种,B簇为贵州省油料研究所选育的2个品种。 A簇在相似系数0.6434处又分为 A1和 A22个亚簇,A1亚簇为贵州省油菜研究所选育的3个品种,A2亚簇为贵州省油料研究所选育4个品种。[结论]同一单位选育出的品种都有更为接近的遗传背景;在该研究所用品种中,贵州省油料研究所选育的品种遗传基础相对较广,但黔油17与黔油29的遗传相似性系数达0.87,遗传基础较为相近。

英文摘要:

[Objective] The aim was to investigate the genetic diversity of the main commercial varieties of Brassica napus in Guizhou Province at the molecular level. [Method] Nine main commercial rapeseed cultivars in Guizhou were detected by 40 pairs of SSR primers used in the national regional trial of B. napus, and then clus-tering analysis was carried out. [Result] A total of 191 bands were amplified, and 143 (accounting for 75%) of them showed polymorphism among the nine rapeseed cultivars. By cluster analysis, the nine cultivars were divided into two groups. Group A included seven varieties, and group B consisted of two cultivars from Guizhou In-stitute of Oil Crops. The cultivars in group A were further divided into sub-groups A1 and A2 at the similarity coefficient of 0.643 4. Sub-group A1 included three culti-vars from Guizhou Rapeseed Institute, and sub-group A2 included the other four cultivars from Guizhou Institute of Oil Crops. [Conclusion] The cultivars bred by the same institute had similar genetic background. The cultivars from Guizhou Institute of Oil Crops showed wider genetic basis. However, the genetic similarity coefficient between Qianyou 17 and Qianyou 29 was up to 0.87, suggesting that they shared closer genetic basis.

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期刊信息
  • 《机床与液压》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学技术协会
  • 主办单位:中国机械工程学会生产工程分会 广州机械科学研究院
  • 主编:闵新和
  • 地址:广州市黄埔区茅岗路828号
  • 邮编:510700
  • 邮箱:jcy@gmeri.com
  • 电话:020-32387859
  • 国际标准刊号:ISSN:1001-3881
  • 国内统一刊号:ISSN:44-1259/TH
  • 邮发代号:46-40
  • 获奖情况:
  • 2011荣获第四届广东省优秀科技期刊一等奖2010年...
  • 国内外数据库收录:
  • 中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:28254