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中文摘要：
目的探讨顺铂（DDP）预处理对细胞因子诱导的杀伤细胞（CIK）杀伤活性的影响。方法采用CFSE/PI双标法检测DDP预处理不同浓度、不同时间及预处理前后不同效靶比,CIK对肺癌细胞的杀伤活性。结果经IFN-γ,CD3单抗,IL-2诱导后20d,获得典型的CIK细胞做为效应细胞。随着DDP预处理浓度的增加,CIK细胞的杀伤活性随之增强,随着DDP预处理时间的延长,CIK细胞的杀伤活性亦随之增强。在DDP预处理浓度25ng/ml,预处理时间18h的条件下,DDP对靶细胞并无明显影响,但联合CIK后,随着效靶比的增高,CIK细胞杀伤肿瘤细胞的活性明显增强。结论顺铂预处理肺癌细胞后,提高了CIK细胞的杀伤活性,对CIK细胞的临床应用具有借鉴意义。
英文摘要：
Objective To study the cytotoxic effect of Cisplatin pretreated on cytokine-induced killer（CIK） against lung cancer cells.Methods Lung cancer cell line A549 and H446 were used in the present study.CFSE/PI double labeling method was used to detect the cytotoxic effect of CIK after Cisplatin pretreatment lung cancer cell line A549 and H446 at different concentration,different time and different CIK effect/target ratios.Results We succeed cultivate CIK as effect cells.With the increase of DDP precondition concentration,the cytotoxic effect of CIK increased.With the prolong of DDP precondition time,the cytotoxic effect of CIK increased,and after DDP precondition target cells 18 h at the concentration of 25 ng/ml,it has no significant difference on target cells,but with the effect/target ratio increase,the cytotoxic effect of CIK increased significantly compared to the without DDP precondition one.Conclusion Preconditioning lung cancer cell with Cisplatin can enhance the cytotoxic effect of CIK.This study might provide valuable information on the clinical application of CIK cells.
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