中文摘要：

Orexin 是影响家畜的胃口和食物吸入的重要 neuropeptide 并且仔细与家畜的生长和发展被联系。在 orexin 基因的变化 5 规章的区域可能在基因表示上有影响。基于假设，五重叠了包括 orexin 基因 5 规章的区域的 1 794 bp 的碎片被在三张土生土长的牛人口定序的 PCR-SSCP 和 DNA 为单个核苷酸多型性(SNP ) 调查。观察的五个 SSCP 模式的一个总数在这个区域揭示了十 SNP。二 SSCP 模式遗传型(A 和 B ) 在 0-2 碎片被展出，(A， B 和 C ) 三在 0-5 碎片被发现。0-2 包含了四 SNP， viz， 583 T > C， 479 OT， 474 A > T，和 451 A > G。在另一 lous 0-5，六 SNP 被识别(-1 610 OG， 1 585 G > 一， 1 550 T > C， 1 548 A > C， 1 438 OT，和 1 431 OA ) 。七 SNP 在抄写因素绑定地点被发现，四在核心序列从他们存在。在 479， 474 和 451 点的 SNP 没改变他们的因素的通常认为的识别核心序列。但是在 583 点的变化把 EVI1 的有约束力的顺序变成了 NFA 并且同时为 ZFHX 创造了一个新有约束力的地点。在三张人口， A 的频率， B 和 C， 0-2 的遗传型分别地是 0.2367， 0.4842 和 0.2791。并且 0-5 的 A 模式是优势的(0.7549 ) 并且另外的 B 模式不是(0.2451 ) 。但是不同 SSCP 变体的频率越过三张牛人口变化了。在在 Nanyang 牛 6-mon 和 12-mon 的到身体重量(BW ) 和每日的重量的有的重要协会增加的 0-2 (DWG ) 的 B 遗传型变老并且可能用作一个潜在的候选人为生长和开发的基因标记。

英文摘要：

Orexin is an important neuropeptide that influences livestock＇s appetite and food intake and is closely related with livestock＇s growth and development.The variations in the orexin gene 5＇ regulatory region might have an influence on the gene expression.Based on the hypothesis,five overlapped fragments including 1 794 bp of orexin gene 5＇ regulatory region were investigated for single nucleotide polymorphisms （SNPs） by PCR-SSCP and DNA sequencing in three indigenous cattle populations.A total of five SSCP patterns observed revealed ten SNPs in the region.Two SSCP patterns genotypes （A and B） were exhibited in O-2 fragment and three （A,B and C） were found in O-5 fragment.O-2 contained four SNPs,viz.,-583 TC,-479 CT,-474 AT,and-451 AG.In another lous O-5,six SNPs were identified （-1 610 CG,-1 585 GA,-1 550 TC,-1 548 AC,-1 438 CT,and-1 431 CA）.Seven SNPs were found in transcription factor binding sites and four out of them existed in the core sequences.The SNPs at-479,-474 and-451 did not change the putative recognition core sequences of their factors.But the mutation at-583 changed the binding sequence of EVI1 into NFA and created one new binding site for ZFHX simultaneously.In three populations,the frequencies of A,B and C genotypes of O-2 were 0.2367,0.4842 and 0.2791,respectively.And the A pattern of O-5 was preponderant （0.7549） and the other B pattern was not （0.2451）.But the frequencies of different SSCP variants varied across three cattle populations.B genotype in O-2 had significant associations to body weight （BW） and daily weight gain （DWG） in Nanyang cattle 6-and 12-mon aged and might serve as one potential candidate genetic marker for growth and development.