中文摘要：

目的观察P2X，受体拮抗剂亮蓝G（brilliantblueG，BBG）在颈上神经节P2X，受体介导心肌缺血引发的交感兴奋反射信息传递中的作用，为冠心病的防治提供新的治疗靶点。方法左冠状动脉结扎建立心肌缺血大鼠模型，检查动物心电图了解心功能的变化；使用无创法血压计测量大鼠的心率和血压的变化；用全自动电化学发光仪检测大鼠血清乳酸脱氢酶（LDH）、肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）和心肌肌钙蛋白T（cTn—I）的含量；免疫组织化学法和蛋白印迹（Westernblot）观察颈上神经节的P2X，受体蛋白的表达情况。结果大鼠心肌缺血20d后心电图出现深大的病理性Q波，心率加快、收缩压和舒张压增高，BBG干预后大鼠心电图的异常变化得到改善、加快的心率减慢、增高的收缩压和舒张压降低。心肌缺血模型大鼠的LDH、CK—MB、CK和cTn—I浓度明显增加，BBG降低增高的血清心肌酶。心肌缺血模型组大鼠颈上神经节P2X，受体免疫反应性和蛋白水平明显高于假手术组，BBG明显降低增高的P2X，受体水平。结论大鼠颈上交感神经节P2X，受体介导心肌缺血损伤性交感兴奋反射过程，P2X，特异性拮抗剂BBG降低P2X，表达，抑制心肌缺血损伤性颈部交感神经兴奋反射，进而产生心肌保护作用。

英文摘要：

Aim To observe the effects of P2X7 antag- onist brilliant blue G （BBG） on the nociceptive signa- ling of sympathoexcitatory reflex induced by myocardial ischemic injury via P2X7 receptor of superior cervical ganglia（SCG） and look for new effective targets for the preventive and therapeutic action of coronary heart dis- eases. Methods The left anterior coronary arteries of rats were ligated and then used as myocardial ischemic injury model. The changes of cardiac function in myo- cardial ischemic injury rats were observed by electro- cardiogram （ECG）. The heart rates and blood pres- sure were measured via non-invasive blood pressure de- terminator. Lactate dehydrogenase （LDH）, creatine kinase （CK）, creatine kinase isoform MB （CK-MB） and cardiac troponin I （cTn-I） were tested by full-au- tomatic flash instrument of electricity and chemistry. The expression of P2X7 immunoreactivity and protein in rat SCG was checked by immunohistoehemistry and western blotting. Results There was an abnormal Q wave in myocardial ischemic rats after 20 days of myo- cardial ischemia. The heart ra te and blood pressure （systolic blood pressure and diastolic blood pressure） in the myocardial ischemic rats were enhanced. After treated with BBG, abnormal changes in ECG induced by myocardial ischemia were improved and the upregu- lated heart rate and blood pressure in the myocardial ischemic rats were reduced. LDH, CK, CK-MB and cTn-I in the blood serum of myocardial ischemic rats were enhanced, which were decreased by BBG. The expression levels of P2X7 immunoreactivity and protein in SCG were significantly higher than that in sham group, which were decreased by BBG. Conclusions P2X7 receptor in SCG is involved in the sympathoexci- tatory reflex induced by myocardial ischemic injury sig- nal. P2X7 antagonist BBG inhibits the nociceptive sig- naling of sympathoexcitatory reflex induced by myocardial ischemic injury and offers the cardioprotective action.