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同型巴蜗牛凝集素的凝集活性及影响因素
  • ISSN号:1006-6330
  • 期刊名称:《应用数学与计算数学学报》
  • 时间:0
  • 分类:Q513[生物学—生物化学]
  • 作者机构:[1]泉州师范学院生物系,福建泉州362000, [2]泉州医学高等专科学校,福建泉州362000, [3]集美大学水产学院,福建厦门361021
  • 相关基金:福建省教育厅省属高校科研专项(Jk2010050);福建省自然科学基金资助项目(2008J04002);泉州市科技局技术研究与开发项目(2008N41);福建省高校服务海西建设重点项目(A101)
中文摘要:

对同型巴蜗牛(Bradybaena similaris)蛋白腺提取液凝集活性进行测定,初步提取其凝集素并进行部分性质鉴定.结果表明:在同型巴蜗牛的蛋白腺中存在凝集素,具有血球凝集活性,能够凝集9种动物红细胞,其中,对家兔红细胞的凝集活性最高,可达2^8,不过,其对家兔红细胞的凝集活性明显依赖于Ca2+;在pH值为7.0~8.0的范围内其凝集活性较稳定,保持100%的凝集活力;温度为40℃持续作用10min后就失去活性;向同型巴蜗牛蛋白腺提取液加人45%的固体硫酸铵使凝集素沉淀,再用不同饱和度的硫酸铵溶液依次对沉淀物进行抽提,抽提物的比活力提高了约2.56倍,总活力回收为20.00%.

英文摘要:

The agglutination activity of albumen gland extract from a Bradybaena similaris was measured. The agglutinin was extracted and part of its properties was identified. The results showed agglutinin existed in the albumen gland of bradybaena similaris ; this agglutinin had blood-cell agglutination activity, and was able to agglutinate 9 kinds of animal red blood-cells, of which the domestic rabbit's was agglutinated with highest activity up to 2s. However, the agglutination activity on domestic rabbit's red blood - cell was significantly dependent on Ca2+. It was stable in pH 7.0 -8.0 with 100 % activity, and was lost after continuous functio-ning for 10 minutes in 40 ℃ temperature, which indicated its heat resistance was poor. 45 % solid ammonium sulfate was added into the albumen gland extract from a bradybaena similaris to precipitate the agglutinin, and then the precipitates were extracted respectively by using ammonium sulfate solution with different saturation. The specific activity of the extract was. increased by about 2. 56 times with total activity recovery of 20. 00%.

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期刊信息
  • 《应用数学与计算数学学报》
  • 主管单位:上海市教育委员会
  • 主办单位:上海大学
  • 主编:马和平
  • 地址:上海市上大路99号121信箱上海大学期刊社
  • 邮编:200444
  • 邮箱:camc@oa.shu.edu.cn
  • 电话:021-66137602
  • 国际标准刊号:ISSN:1006-6330
  • 国内统一刊号:ISSN:31-1436/O1
  • 邮发代号:
  • 获奖情况:
  • 国内外数据库收录:
  • 美国数学评论(网络版),德国数学文摘
  • 被引量:1282