中文摘要：

目的观察白细胞介素-17（IL-17）对HaCaT细胞分泌血管内皮生长因子（VEGF）的影响及紫草素的干预作用。方法收集IL-17刺激组和紫草素处理后HaCaT细胞及其培养上清，分别用双抗体夹心酶联免疫吸附法（ELISA）及实时荧光免疫定量-聚合酶链反应法（RT-PCR）检测VEGF含量。应用细胞计数盒-8（CCK-8）检测各组HaCaT细胞活力。结果与对照组相比，不同时间IL-17刺激组HaCaT细胞及其培养上清中VEGF表达均高于对照组（P〈0．001）；紫草素处理组HaCaT细胞及其培养上清VEGF表达均低于IL-17处理组（P〈0．001）；与对照组比较，CCK-8检测各组HaCaT细胞活力无明显差异（P〉0．05）。结论IL-17可促进HaCaT细胞分泌VEGF，呈时间依赖型，而紫草素对其具有抑制作用。

英文摘要：

Objective To investigate whether IL-17 could stimulate the vascular endothelial growth factor （VEGF） production on HaCaT cells alone. We also investigated whether shikonin could inhibi- ted the proinflarnation effects of interleukin-17 （IL-17） acting on HaCaT cells. Methods We examined the expression of VEGF by double antibody sandwich enzyme-linked immunosorbent assay （ELISA） and real- time polymerase chain reaction（RT-PCR） in HaCaT cells and the cell supernatant. The viability of HaCaT cells in the drug group was detected by the Cell Counting Kit-8 （ CCK-8 ）. Results The expression of VEGF in different time IL-17-stimulated groups on HaCaT cells and the cell supernatant were higher than the con- trol group（P〈0.001 ）. The expression of VEGF in different drug treatment groups on HaCaT cells and the cell supernatant were lower than the stimulated group by IL-17 （ P〈0. 001 ）. The cell viability of different drug treatment groups have no significant difference（P〉0.05）. Conclusion We show that IL-17 specific- ally and time-dependently augmented and induced VEGF expression on HaCaT cells and the cell supernatant Then shikonin markedly inhibited the increase tengency of IL-17 effection on HaCaT cells and the cell supernatant level.