中文摘要：

目的 探讨黄芩素、阿维A对人皮肤鳞状细胞癌细胞株SCL-12细胞凋亡的影响.方法 不同浓度黄芩素、阿维A单独及联合作用于SCL-12细胞,噻唑蓝（MTT）法检测上述药物对SCL-12细胞的生长抑制作用;膜联蛋白V-异硫氰酸荧光素/碘化丙啶（Annexin V-FITC/PI）双染法和ELISA法检测SCL-12细胞凋亡率;实时荧光定量RT-PCR法检测Fas mRNA的表达.结果 黄芩素、阿维A单独及联合应用均可抑制SCL-12细胞的生长,除3.125 μmol/L黄芩素与2.5 μmol/L阿维A联合用药组外,其余各联合用药组与单药作用组相比,抑制作用明显增强,且抑制作用随用药浓度的增加而增强.3.125 μmol/L黄芩素、5.0 μmol/L阿维A单独及联合应用48 h后均可诱导SCL-12细胞凋亡,早期凋亡率分别为9.39%±1.52%、20.86%±2.16%、36.85%±3.26%,与对照组（4.39%±0.64%）比较,差异均有统计学意义（P值均〈0.05）;联合用药组对SCL-12细胞凋亡的诱导作用显著强于单药作用组,差异有统计学意义（F=138.44,P〈0.05）.黄芩素、阿维A单独及联合应用48 h后均可上调SCL-12细胞Fas mRNA表达水平,联合用药组SCL-12细胞的Fas mRNA表达水平显著高于单药作用组.结论 黄芩素、阿维A单独及联合应用均能抑制SCL-12细胞生长并诱导其凋亡,两种药物的联合应用对SCL-12细胞具有协同效应,其发生机制可能与上调Fas凋亡调控基因的表达有关.

英文摘要：

Objective To investigate the effects of baicalein and acitretin on the apoptosis in a human cutaneous squamous cell carcinoma cell line, SCL-12. Methods Cultured SCL-12 cells were treated with different concentrations of baicalein （3.125, 6.25, 12.5 μmol/L） and acitretin （2.5, 5.0, 10.0 μ mol/L）, alone or in combination, for 48 hours. Subsequently, cell proliferation was detected by MTT assay, and cell apoptosis by ELISA as well as annexin V-FITC and propidium iodide double staining. Real-time quantitative RT-PCR was used to detect the expression of Fas mRNA in SCL-12 cells. Results The cell proliferation of SCL-12 cells was inhibited by baicalein and acitretin alone or in combination. The combination of baicalein and acitretin at the three tested concentrations, except for that of baicalein at 3.125 μmol/L and acitretin at 2.5 μmol/L, more strongly inhibited the proliferation of SCL-12 cells compared with baicalein or acitretin alone, and the inhibitory effect was in a dose-dependent manner. The early apoptosis rate was 9.39% ± 1.52%, 20.86% ± 2.16%,36.85% ± 3.26% in SCL-12 cells treated with baicalein of 3.125 μmol/L, acitretin of 5.0 μmol/L alone and their combination, respectively, significantly higher than that in untreated cells （4.39% ± 0.64%, all P 〈0.05）; the induction of apoptosis in SCL-12 cells by the combination of baicalein and acitretin was stronger than that by baicalein or acitretin alone （F = 138.44, P 〈 0.05）. Baicalein and acitretin alone or in combination significantly increased the mRNA expression of Fas in SCL-12 cells, and the effect of their combination was stronger than that of baicalein or acitretin alone. Conclusions Baicalein and aeitretin could inhibit the growth of and induce the apoptosis in SCL-12 cells, and the effect is enhanced by the combination of baicalein and acitretin, which may be associated with the upregulation of Fas expression in SCL-12 cells.