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中文摘要：
目的研究寻常型天疱疮（PV）特异性抗原桥粒芯糖蛋白3（Dsg3）刺激下PV患者一级亲属T淋巴细胞的增殖情况,探讨PV的发生机制。方法Dsg3刺激PV患者一级亲属外周血单个核细胞（PBMC）体外培养3 d,BrdU掺入流式细胞术胞内染色,检测Dsg3特异性CD3＋、CD3＋CD4＋、CD3＋CD8＋T细胞的增殖情况。结果PV抗体阳性亲属中PBMC经Dsg3刺激培养者与未经Dsg3刺激培养者比较,CD3＋CD4＋T细胞增殖率显著升高[（2.79±1.05）%vs（1.85±0.44）%,P〈0.05],PV抗体阳性亲属CD3＋CD4＋T细胞增殖率明显高于正常对照者[（2.79±1.05）%vs（1.25±0.71）%,P〈0.05]。结论PV患者一级亲属中PV抗体携带者体内存在Dsg3自身反应性CD3＋CD4＋T淋巴细胞,Dsg3释放可能在PV启动中起重要作用。
英文摘要：
Objective To investigate the characteristics of T lymphocyte proliferation in first-degree relatives of the pemphigus vulgaris（PV）patients under the stimulation of Dsg3 and clarify the mechanism of the Dsg3-specific autoimmunity of PV.Methods We examined Dsg3 reactive peripheral blood T-cell subsets CD3＋,CD3＋CD4＋,CD3＋CD8＋ T cells by BrdU incorporation assay and flow cytometry.Results After stimulated by Dsg3 antibody,the proliferative ratio of CD3＋CD4＋T lymphocytes in first-degree relatives with PV antibody positive was significantly increased [ （2.79±1.05）% vs （1.85±0.44）%, P 〈 0.05 ]. Dsg3-speciilc CD3＋CD4＋ T lymphocyte proliferation on PV-antibody- positive first degree relatives was significantly higher than that of healthy controls [ （2.79±1.05）% vs （ 1.25±0.71 ）%, P 〈 0.05 ]. Conclusion The first-degree relatives of PV patients who carry the PV antibodies have Dsg3 autoreative T lymphocytes. The release of Dsg3 may play important roles in the initiation of PV.
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