欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的探讨不同分子亚型副溶血弧菌(Vibrio parahaemolyticus,Vp)的生长动力学特征,为建立海产品中Vp预测模型提供数据。方法应用PCR和RAPD方法对5株不同来源的Vp分离株进行了分子鉴别,并对不同菌株在适宜温度(37℃)和低温(15℃)下生长变化进行了测定,采用修正的Gompertz方程拟合细菌的生长曲线,求出不同菌株的生长动力学参数并建立预测模型。结果4株食物中毒样品来源的Vp菌株基因型均为tl+tdh+trh-toxR+,1株海产品分离株为tl+tdh-trh-toxR+,筛选的随机引物P21可以将5株不同来源的Vp菌株分成5个不同的分子亚型,不同菌株37℃的世代时间在0.210~0.238h之间,延滞时间在1.705~1.849h之间,生长曲线没有显著差异(R2>0.995),应用预测模型求得各菌株的预测值与实测值均方根偏差在0.110~0.452之间;而在15℃条件下测得不同Vp菌株世代时间在0.727~1.062h之间,延滞时间在10467~12552h之间,生长曲线具有较大的变异性(R2>0.938),其中菌株VP06003和VP06127的预测值与实测值之间均方根偏差分别为...
英文摘要：
To characterize growth kinetics model of different subtypes of Vibrio parahaemolyticus and to provide useful data for developing risk assessment of V. parahaemolyticus in seafood, PCR and RAPD techniques were applied to molecular characterization of 5 strains of V. parahaemolyticus isolated from different sources and the growth of bacterial at 37℃ and 15℃ were analyzed. Growth curves were fitted using modified Gompertz equation and kinetic parameters of different strains and predictive model were developed ...
同期刊论文项目
同项目期刊论文
Listeria monocytogenes in the Chinese food system: strain characterization through partial actA sequ
Isolation?and?molecular?characterization?of?Vibrio?parahaemolyticus from?fresh,?low-temperature?pres
Priming with a DNA vaccine delivered by attenuated Salmonella typhimurium and boosting with a killed
Changes in antimicrobial resistance among Salmonella enterica subspecies enterica serovar Pullorum i
Distribution, prevalence, molecular typing, and virulence of Vibrio parahaemolyticus isolated from d
位置：