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中文摘要：
以含有鸡白细胞介素2（ChlL-2）基因的重组真核表达质粒pcDNA3．1-ChlL-2免疫6周龄BALB／c小鼠，末次加强免疫后取其脾细胞与骨髓瘤细胞Sp2／0-Ag-14融合，应用间接ELISA筛选阳性克隆。共获得2株特异性分泌抗鸡IL-2分子的杂交瘤细胞株，分别命名为1H10和1E6，其腹水ELISA效价分别为1：6400和1：3200，亚类鉴定结果均为IgM。重组质粒pcDNA3．1-ChlL-2转染COS-7细胞，以上述单克隆抗体（mAb）检测表达产物，结果表明，2株mAb均能与表达产物反应；Western blot分析结果显示，2株mAb均与原核表达鸡IL-2蛋白发生特异性反应。本研究所制备的抗鸡IL-2分子mAb可为建立简单、快速的鸡IL-2检测方法提供可能，并为鸡IL-2生物学特性和禽类细胞免疫机理的研究提供材料。
英文摘要：
Sp2/0-Ag-14 myeloma cells was fused with splenocytes from BALB/c mice immunized against chicken IL-2 and the supernatant of hybridoma clones were screened by indirect ELISA. Two hybridoma cell lines 1H10 and 1E6 secreting moloc-lonal antibodies （Mab） against ChlL-2 were obtained. The mAbs had an ascites titers pf 1：6 400 and 1：3 200 respectively and both were subtype IgM. IFA assay revealed that both mAbs could react with COS-7 cells transfected by pcDNA3.1-ChlL-2. Western blot analysis confirmed that these mAbs specifically recognized ChlL-2 only. Therefore the McAb developed in this work could be a useful tool to study avian immune cell function and immune regulations, and used as an effective reagent for the detection of chicken IL-2.
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