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中文摘要：
应用RT—PCR技术，从被诱导的鸡脾脏淋巴细胞中扩增鸡白细胞介素-4（ChIL-4）基因cDNA，经克隆筛选和测序后，构建出重组质粒pGEX-6P—ChIL-4和pET—ChIL-4，分别转化相应的受体菌，经IPTG诱导和SDS—PAGE分析，结果表明ChIL-4基因在上述载体中均获得表达，融合蛋白大小分别为38ku和18ku．Western blot结果也显示，在相对分子质量38ku和18ku位置有特异性条带。这为重组ChIL-4的规模化生产、疫苗佐剂的研制及其单克隆抗体的制备奠定了基础。
英文摘要：
Chicken IL-4 gene cDNA was amplified by RT- PCR fi-om chicken spleen lymphocytes stimulated by concanavalin A （ConA）, Recombinant expression plasmid pGEX-6P-ChIL-4, pBCX-ChlL-4, pET-ChlL-4 were constructed and expressed in E, coli by IPTG induction. SDSPAGE analysis showed that the expression products were fusion proteins with molecular weight of 38 ku, 45 ku, 18 ku, respectively, These constructs could be a useful resource for the production of ChIL-4, vaccine adjuvant and monoclonal antibodies development against chicken IL-4.
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