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沙门氏菌gyrA基因的变异对氟喹诺酮类药物敏感性的影响

期刊名称：中国人兽共患病学报，2007，23(9)：891-894
时间：0
分类：R378.2[医药卫生—病原生物学;医药卫生—基础医学]
作者机构：[1]江苏省人兽共患病学重点实验室,扬州225009, [2]扬州大学农业部畜禽传染病学重点开放实验室, [3]扬州市疾病预防控制中心
相关基金：国家杰出青年科学基金资助项目（30425031）,教育部FANEDD专项基金（200358）
相关项目：兽医传染病学

关键词：萘啶酮酸耐药, 沙门氏菌, 氟喹诺酮敏感性, gyrA变异, gyrA gene mutation, susceptibility to fluoroquinolones, Salmonella

中文摘要：

目的分析125株萘啶酮酸耐药性沙门氏菌gyrA基因QRDR区变异情况及其对氟喹诺酮类药物敏感性的影响。方法用常规血清学方法鉴定沙门氏菌分离株血清型，通过微量稀释法测定其对萘啶酮酸和3种氟喹诺酮的敏感性，用PCR方法扩增沙门氏菌gyrA基因QRDR的序列并进行序列分析。结果这些分离株涉及18个血清型，最常见的血清型为德尔卑沙门氏菌和肠炎沙门氏菌，它们的分离率分别为22.4%（28/125）和19.2%（24/125）。沙门氏菌分离株对萘啶酮酸的耐药率为39/125。3种氟喹诺酮药物对萘啶酮酸耐药性沙门氏菌的最小抑菌浓度明显比萘啶酮酸敏感株高。萘啶酮酸耐药性沙门氏菌gyrA基因QRDR的序列分析表明，所有萘啶酮酸耐药性沙门氏菌gyrA基因的83或87位点发生了突变，83位点发生点突变的菌株对萘啶酮酸的MIC范围大于或等于1024μg/ml，而87位点突变的菌株的MIC范围为32～512μg/ml。结论沙门氏菌分离株gyrA基因发生点突变的位点与萘啶酮酸耐药水平高低存在关联，且导致对三种氟喹诺酮类药物的敏感性下降。

英文摘要：

The goal of this study was to analyse the effect of gyrA gene mutation in Salmonella on the drug-susceptibility to fluoroquinolones, in which the mutation of QRDR region of gyrA gene in 125 strains of Salmonella resistant to nalidixic acid isolated from humans and foods from 2003 to-2006 was analyzed. The serotypes of the Salmonella isolates were identified by routine serological methods and their susceptibility to nalidixic acid and 3 fluoroquinolones was determined by broth microdilution method. It was found that these isolates involved with 18 different serotypes, in which the most common ones were Sl. derby and Sl. enteritidis with the detection rates of 22.4% （28/125） and 19.2% （24/125） respectively. These isolates were susceptible to ciprofloxacin except one isolate, but exhibited reduced susceptibility to 3 fluoroquinolones. The drug-resistance rate to nalidixic acid of these isolates was 39/125, and the minimal inhibitory concentration （MIC） of 3 fluoroquinolones on isolates resistant to nalidixic acid was significantly higher than those of the sensitive strains. From the analysis on the QRDR sequence of gyrA gene in Salmonella strains resistant to nalidixic acid, it was demonstrated that point mutations occurred at positions 83 and 87 in gyrA gene of all nalidixic acid resistant strains of Salmonella, in which the MIC range to nalidixic acid of isolates with point mutation at position 83 was more than or equal to 1 024μ/ml, but that at position 87 was 32-512 μg/ml. The result obtained in this study illustrates the simultaneous presence of nalidixic acid resistance and decreased ciprofloxacin susceptibility in Salmonella isolates, and that this resistance mainly associates with mutations in gyrA gene.

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