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中文摘要：
用Trizol分别提取4～6周龄白莱航鸡、狼山鸡、大骨鸡、北京油鸡、仙居鸡、茶花鸡、固始鸡和隐性白羽鸡胸腺细胞的总RNA，再用Oligo-dT纤维索富集mRNA后，用RT-PCR分别扩增出鸡CD4和cD8α基因cDNA。将PCR产物克隆进T载体后测序。序列分析显示不同品种鸡的CD4 cDNA序列完全一致；中国地方品种鸡与国外品种的白莱航鸡、RPL7系鸡在CD8α胞外区有6～13个氨基酸的差异，仙居鸡与其他5个中国地方品种鸡在CD8α胞外区有4～5个氨基酸的差异，狼山鸡、北京油鸡和隐性白羽鸡CD8α cDNA的核苷酸序列及推导的氨基酸序列完全一致。这些结果表明，中国地方品种鸡CD4基因序列高度保守；中国地方品种鸡与国外品种鸡的CD8α cDNA序列之间具有遗传多态性，而中国地方品种鸡之间CDSα cDNA序列遗传多态性低，为进一步研究CD4分子和CD8α链的结构和功能提供了条件。
英文摘要：
Total thymocyte RNAs were extracted from 4-6 week old White Leghorn, Langshan, Bigbone, Beijing fatty chicken, Camellia, Xianju and Recessive white feather chickens respectively, mRNAs were isolated from total RNAs using oligo-dT cellulose, then CD4 or CD8α cDNAs were amplified with RT-PCR using the purified mRNAs as the templates. The cDNAs were sequenced after inserted into Promega T easy vector. The sequencing analysis showed that the CD4 sequences of different breeds shared high homology, the CD8α sequences of Chinese local breeds had 6 13 amino acid differences at extracellular domain as compared with those of White Leghorn and RPL line 7. Among Chinese local breeds tested, CD8α sequence of Xianju chicken had 4 -5 amino acid differences at extracellular domain as compared with others, while Langshan, Beijing fatty chicken and Recessive white feather chickens share the same sequence of CD8α cDNA. It was shown that the CD4 sequences of Chinese local breeds are highly conserved, while CD8α sequences have lower polymorphism than those of foreign breeds. These results provided useful information for further study on the structure and function of CD4 and CD8 molecules.
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