欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
以16SrRNA、mapA和ceuE为靶基因，建立检测空肠弯曲菌和结肠弯曲菌的多重PCR方法。特异性试验能分别扩增出空肠弯曲菌和结肠弯曲菌的特异性条带，其他参考菌株均未扩增出条带；敏感性试验表明，基于增菌培养和选择性培养的多重PCR方法对弯曲菌检测限为10mL或10g模拟污染样品，最低可检测0．925Pg·uL^-1空肠弯曲菌DNA和1．050Pg·uL^-1结肠弯曲菌DNA。以国家标准为参照，应用该方法对180份生鲜鸡肉样品进行检测，结果表明多重PCR方法具有快速、简便、特异性强和灵敏度高等特点，可克服传统生化鉴定和血清学方法的不足，为弯曲菌检测提供新的技术。
英文摘要：
A multiplex PCR （m-PCR） targeting 16,5 rRNA gene, mapA gene and ceuE gene for the detection of Campylobacter jejuni and Campylobacter coli was developed. Species-specific product could be detected after amplification of the DNA template of C. jejuni and C. coli, while other bacteria strains could not be detected. The m-PCR assay could detect as low as 10 mL or 10 g, artificial contaminated samples and its detect limit of C. jejuni and C. coli were 0. 925 and 1. 050 pg · uL^-1 DNA template respectively. 180 of fresh chicken .samples were detected for the C. jejuni and C. coli by the m- PCR, and the result was coincided with that of the national standard method for Campylobacter. It was suggested that the m-PCR was highly specific and sensitive, and could overcome the shortcomings of biochemical and serological identification.
同期刊论文项目
同项目期刊论文
Listeria monocytogenes in the Chinese food system: strain characterization through partial actA sequ
Isolation?and?molecular?characterization?of?Vibrio?parahaemolyticus from?fresh,?low-temperature?pres
Priming with a DNA vaccine delivered by attenuated Salmonella typhimurium and boosting with a killed
Changes in antimicrobial resistance among Salmonella enterica subspecies enterica serovar Pullorum i
Distribution, prevalence, molecular typing, and virulence of Vibrio parahaemolyticus isolated from d
期刊信息
位置：