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中文摘要：
应用抑制差减杂交技术（SSH）对鸡白痢沙门氏菌C79—13与肠炎沙门氏菌50041进行基因组片段的差异分析，构建鸡白痢沙门氏菌的差减文库。经Dot—blot筛选，并对部分片段进行测序和同源性分析。结果表明：这些序列主要分为3类，即Ⅱ型分泌系统相关序列、质粒转移相关序列、未知功能序列。其中2个差减片段与编码福氏志贺菌侵入质粒抗原IpaJ蛋白基因的同源性达50％。证明所构建的差减文库可为鸡白痢沙门氏菌特异性致病基因和其致病机理的研究提供重要的依据。
英文摘要：
Using suppression subtractive hybridization （SSH）, the Salmonella pullorum subtractive library was constructed by comparison of genomic differences between Salmonella pullorum C79-13 （tester） and Salmonella enteritidis 50041 （driver）. Parts of subtracted fragments were sequenced and searched homologically in GenBank after identification by Dot-blot. The results showed that three types of fragments were identified： type I secreton sequences, sequences for plasmid transfer, sequences with currently unknown function. The amino acid homology of two subtracted sequences with ipaJ of Shigella flexneri was 50%. The results suggested that the S. pullorum subtracted library was an important material for searching pathogenicity-related sequences and analyzing pathogenesis of Salmonella pullorum.
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