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中文摘要：
以鲶鱼骨蛋白为原料制备ACE抑制肽，采用碱性蛋白酶、中性蛋白酶、木瓜蛋白酶、胰蛋白酶、胃蛋白酶进行酶解，通过体外检测法测定其抑制率，优选出最佳用酶。通过单因素试验和正交试验，确定了碱性蛋白酶酶用量、pH、酶解温度、酶解时间、底物浓度等因素对ACE活性抑制效果的影响。结果表明：利用碱性蛋白酶水解鲶鱼骨，酶用量为500U／g、pH值8．5、温度为60℃、底物浓度为0．33kg／L、时间6h时酶解液对ACE的抑制活性最强，抑制率为88．36％。通过比较，最佳酶解条件下得到的降血压肽的ACE抑制率要高于降血压药尼莫地平和硝苯地平的ACE抑制率。
英文摘要：
Using catfish bone, the antihypertensive peptides was produced. The catfish was hydrolysised by Alkaline Protease , Neutral Protease, papain, Trypsin, pepsin and ACE Inhibition rate was detected using detection method in Vitro. The optimal Enzyme was selected. This paper showed the effet of Alkaline Protease addition, pH, tempetat- ure, time, substrate concentration to ACE Inhibitory activity. The result was as followed： ACE Inhibitory activity was optimal using Alkaline Protease when Alkaline Protease addition was 500U/g, pH value was 8.5, tempetature was 60~C, substrate concentration was 0.33kg/L, time was 6h and ACE Inhibition rate was 88.36%. The ACE Inhibition rate was Higher than Nimodipine and Nifedipine when Enzymatic hydrolysis condition was best.
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