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中文摘要：
为葡萄霜霉病（Plasmopara viticola）的生物防治提供更多的菌种资源,本研究以新鲜的无病害葡萄叶片为试验材料,通过稀释平板分离法分离得到87株真菌。采用平板对峙法进行初筛得到4株对辣椒疫病菌有拮抗作用的菌株,经过叶盘法复筛得到一株防治效果比较好的Y29菌株,其防治效果达到了72.9%,且防效稳定。通过观察Y29菌株的菌落形态、菌丝形态和产孢结构,分析其26S rDNA D1/D2区序列和系统发育树,鉴定其为小光壳属中的Leptosphaerulina australis。
英文摘要：
To provide more resources for the biological control of grape downy mildew, the antagonistic fungi against the disease were screened in this study. By exploiting streak plate method, 87 fungous strains from the health grape leaves were separated. After primary screening by a dual culture technique, 4 strains exhibiting antagonistic activities against pepper phytophthora blight were screened. By method of the leaf disc for secondary screening, Y29 was screened. Y29 had the better inhibitory effect, which the bio-control efficacy was 72.9%. Based on its colony morphology, mycelial morphology, sporogenous structure, D1/D2 region of 26S rDNA sequence and phylogenetie systematics, the Y29 strain was identified as Leptosphaerulina australis.
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