欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
本文利用MTT染色法和点接生物测定法对不同温度干燥处理36h后的葡萄生单轴霉孢子囊存活力和致病力进行了检测,并应用单因素试验和正交试验对其孢子囊进行了MTT染色条件的优化。结果表明：葡萄生单轴霉孢子囊MTT染色的最佳条件为温度36℃、MTT浓度0.05%、染色48h,孢子囊染色率可达83.0%。20℃恒温干燥处理36h显著提高了孢子囊存活力,葡萄生单轴霉孢子囊的蓝色染色率为79.3%,叶片点接发病率为98.9%,显著高于对照的蓝色染色率52.0%和发病率62.7%。MTT染色得到的孢子囊蓝色染色率与点接生物测定法得出的发病率存在很好的线性关系y=1.276 1x-1.939 1,R2=0.996 1,孢子囊的蓝色染色率与叶片点接发病率呈正相关。本研究表明MTT染色法可以用于葡萄生单轴霉孢子囊存活力的快速和准确检测。
英文摘要：
The vitality and pathogenicity of sporangia of Plasmopara viticola after being dried at different temperature for 36 h is detected by using methylthiazolyldiphenyl-tetrazolium bromide（MTT） staining and dropping inoculation bioassay. The single factor test and orthogonal design are performed to optimize the staining conditions of MTT in detecting the vitality of sporangia of P. viticola. It is found that the optimal staining condition of P. viticola sporangia is 48 h dyeing treatment at 36°C in 0.05% MTTsolution, with a staining rate of 83.0%. The vitality of P. viticola sporangia is significantly improved after drying treatment at 20°C for 36 h, and the percentage of blue-stained sporangia is 79.3%, and the disease incidence of grape leaves is 98.9%, being significantly higher as compared with the control with blue staining rate of 52.0%, and disease incidence of 62.7%. The percentage of blue-stained sporangia is positively correlated with the disease incidence, with a formula as y=1.276 1x-1.939 1, R2=0.996 1. It can be concluded that MTT staining method is applicable to a rapid detection of the vitality of P. viticola sporangia.
同期刊论文项目
同项目期刊论文
期刊信息
位置：