以葡萄品种"巨峰"和"美人指"为试材,对葡萄霜霉病菌孢子囊形成和离体萌发的适宜条件进行了研究。结果表明:20℃黑暗、湿度100%并加入2%乳糖为葡萄霜霉病菌孢子囊形成的最适条件;葡萄霜霉病菌孢子囊悬浮液经4℃低温刺激0.5h,用2%乳糖置于15℃黑暗条件下培养,孢子囊萌发率最高。
Taking ‘Jufeng’and ‘Meirenzhi’of grape varieties as materials,the suitable conditions for sporangium formation and germination of Plasmopara viticola were investigated.The results showed that the best formation condition for sporangia of grape downy mildew was at 20℃ under dark and 100% humidity,supplemented with 2%lactose,and the optimal conditions for sporangium germination of P.viticolawas at 15℃under dark and 100%humidity,supplemented with 2%lactose,followed by stimulation of low temperature at 4℃for half an hour.