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中文摘要：
为提高霜霉病高效生防菌株Bacillus cereus BCJB01的发酵水平,通过正交试验、综合优选及糖分流加试验,优化BCJB01的发酵培养基。优化出的培养基配方的质量分数为：玉米粉2.00%、豆粕粉1.00%、四水氯化锰0.003 0%、十二水磷酸氢二钠0.30%、二水磷酸二氢钠0.15%、七水硫酸镁0.035%、氯化钙0.10%和酵母提取物0.30%,接菌后6-12 h流加质量分数0.2%糖。本研究优化的培养基配方在接种量为3%（V/V）,转速为180 r/min,温度为30℃的摇床振荡培养至66 h时,芽孢率近90%,菌体数量达100×10^8cfu/m L以上。
英文摘要：
To improve fermentation level of Bacillus cereus BCJBOI, a highly efficient biocontrol strain against downy mildew, we optimized its fermentative medium through orthogonal test, comprehensive optimization and sugar feeding tests. Mass fraction of the optimized medium formula are 2. 00% corn meal, 1.00% soybean powder, 0. 0030% manganese chloride tetrahydrate, 0. 30% twelve aqueous disodium hydrogen phosphate, 0. 15% sodium dihydrogen phosphate dihydrate, 0.035% magnesium sulfate heptahydrate, 0.10% calcium chloride, 0. 30% yeast extract, 0. 2%sugar added at 6 -12 h after inoculation. It can produce more than 100× 10^8 cfu/mL cells and its spore rate is 90%,when the cultivation conditions are 3% （ V/V） inoculum size, 180r/ min rotation velocity, 30 0C shaky cultivation till 66 hours.
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