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中文摘要：
目的：优选黄独总皂苷的分离纯化工艺。方法：以黄独总皂苷含量为指标，采用紫外分光光度法测定其含量，动态吸附-解吸法筛选大孔树脂型号，并优选黄独总皂苷的分离纯化工艺。结果：D-101型大孔树脂富集纯化黄独总皂苷的最佳工艺为黄独提取液质量浓度0．2g·mL^-1，上样量6BV，流速1．0mL·min^-1，加4BV水洗除杂，50％乙醇4BV解吸，收集洗脱液。此工艺条件下所得提取物黄独总皂苷质量分数61．85％。结论：该优选工艺简单可行，能满足大生产实际要求。
英文摘要：
Objective： To optimize separation and purification technology of total saponins from Dioscorea bulbifera. Method： With the content of total saponins from D. bulbifera as index, which was determined by ultraviolet spectrophotometry, optimum macroporous resin model was selected by dynamic adsorption-desorption method, then separation and purification technology of total saponins from D. bulbifera was optimized. Result： Optimum separation and purification technology conditions of total saponins by D-101 macroporous resin were as follows： the concentration of extraction liquid from D. bulbifera 0.2 g ·mL^-l, sample volume of 6 BV, flow rate of 1.0 mL·min^-1, washed impurity with 4 BV water, and then desorpted with 4 BV 30% ethanol, collected eluate. Under these technology conditions, yield of total saponins from D. bulbifera reached as high as 61.85%. Conclusion： This optimized technology was simple and feasible, which could meet industrial requirements.
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