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中文摘要：
为了研究端粒酶在植物细胞分裂中的作用，探讨端粒酶活性与细胞分裂能力的相关性，本研究以银杏为材料，通过改良的TRAP法分别测定了银杏不同组织器官（种胚、叶片、小孢子、愈伤组织）的端粒酶活性，研究发现具有旺盛分裂能力的愈伤组织具有较高的端粒酶活性。同时以银杏的成熟种胚为材料，采用培养基MS＋1．0mg／L6-BA＋2．0mg／LNAA诱导出银杏的愈伤组织并采用培养基MS＋1．0mg／L6-BA＋2．0mg／LNAA＋0．3mg／L2，4-D＋0．2％活性炭进行继代，检测不同代银杏愈伤组织的端粒酶活性，发现不同代愈伤组织均具有较高端粒酶活性。初代愈伤组织的端粒酶活性较高，但愈伤组织继代初期端粒酶活性降低，而后缓慢升高，恢复并超越初代愈伤组织水平后，端粒酶活性处于一个相对稳定的状态，表明愈伤组织继代培养后期细胞具有旺盛且稳定的分裂增殖能力。
英文摘要：
This study was designed to investigate the relationship between telomerase activity and cell division in Ginkgo biloba L. Telomerase activities in different tissues and organs of G. biloba （embryo, leaf, microspore and callus） were measured respectively using improved TRAP method. The highest telomerase activity was found in embryonal callus, suggesting tissues containing high levels of dividing cells also had high levels of telomerase activity. In addition, the telomerase activities of different generations callus were measured respectively. Callus tissues were induced from the ginkgo embryos and cultured on MS ＋ 1.0 mg/L 6-BA ＋ 2.0 mg/L NAA and MS ＋ 1.0 mg/L 6-BA ＋ 2.0 mg/L NAA ＋ 0.3 mg/L 2,4-D ＋ 0.2% activated carbon medium supplemented with different combinations of plant growth regulators. It had been found that telomerase activities in all generations of callus were high relative to other tissues （leaf, microspore and embryo）. The telomerase activity of the early generation of callus was very high. After initial surge, the telomerase activity decreased in early callus generations, then restored to higher level and stay there. These results indicated that the callus had high and stable telomerase activities in the later period of callus tissue culture.
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