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中文摘要：
MicroRNAs（miRNAs）通过使靶mRNA降解或翻译参与生物体的转录后调控,并在植物对生物与非生物胁迫的应答过程中发挥重要作用。本研究基于相关数据库,利用生物信息学方法,通过搜索预测及实验验证得到甜杨（Populus suaveolens）低温响应miR475的12条潜在靶基因,并对其降解位点进行分析;同时,以低温（0℃）处理不同时间甜杨幼苗为试材,利用荧光定量PCR对miR475及其靶基因的表达谱进行检测分析,结果发现,随着低温诱导时间的延长,miR475表达量呈下调趋势,而其靶mRNA的表达量则呈现相反趋势,表明甜杨miR475可能以降解靶mRNA的方式在抵御低温中发挥作用。本文研究结果可为甜杨抗冻机制的后续深入研究提供科学依据。
英文摘要：
The miRNA participates in posttranscriptional regulation by regulating degradation or translation of target mRNAs, and plays a key roles in response of plants to biotic and abiotic stresses. In this paper, based on the database of mRNA and EST of poplar, the 12 potential target genes of cold-related miR475 were predicted by using bioinformatics and verified in Populus suaveolens, and the cleavage sites of the miRNA target genes were analyzed. In addition, the expression profiles of the obtained target mRNAs and mature miRNA from P. suaveolens seedlings exposed to 0℃ for 0 to 48 hours were analyzed by quantitative realtime PCR.The results reveal that the expression of miR475 was down-regulated and its target mRNAs show the opposite trend, suggesting that miR475 may function in low temperature resistance by regulating degradation of the target mRNAs. The results would provide a theoretical basis to further explore of the molecular mechanism of cold resistance in P. suaveolens.
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