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中文摘要：
在7L发酵罐规模，利用自诱导培养方式，对重组大肠杆菌产普鲁兰酶发酵条件进行优化研究，主要考察了发酵过程中控制溶解氧浓度、pH和采用补料策略对菌体生长和普鲁兰酶表达的影响。结果表明，最佳发酵培养条件：溶氧浓度维持在20％-30％；pH保持在7．60；第9h时，以0．8g／（L·h）的速度流加甘油；在20h，以0．2g／（L·h）的速度流加乳糖。此条件下，重组菌的细胞浓度OD600nm从12．58上升到42．25，提高了3．36倍；普鲁兰酶酶活力从356U／ml上升到1200U／ml，提高了3．37倍。
英文摘要：
By using auto-induction system, the fermentation conditions including oxygen and pH were controlled in a 7-L batch system, and then fed-batch fermentations were carried out using glycerol as a carbon source and lactose as inducer so as to discuss the effects on fermentation by different feeding modes. The optimum conditions were found to be as follows ： the dissolved oxygen concentration was maintained at 20% - 30% , the culture pH was kept at 7.60, and glycerol was added by the rate of 0.8 g/（ L · h） at 9h, lactose was added by the velocity of 0.2 g/（ L · h） at 20 h. Using the optimal conditions, concentration of ceils and activity of pullulanase were increased from 12.58 to 42.25 and from 356 U/mL to 1200 U/mL respectively, which were 3.36 and 3.37 times of those in fed-batch fermentation.
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