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中文摘要：
从堆肥中分离得到一株具有较强拮抗植物病原菌活性的木霉菌,经过分子生物学方法鉴定为侧钩木霉（Trichoderma ghanense）.以该菌为出发菌株,对其固态发酵培养基进行优化,以期提高孢子产量.通过单因素筛选、Plackett-Burman实验、最陡爬坡实验和响应面分析确定影响孢子产量的最重要的三因素为蔗糖、玉米浆、硫酸镁,其最佳浓度分别为0.56％、0.56％和2.59％,最佳初始含水量为35％,最佳初始pH值为9.优化后的固态发酵培养基上,该菌孢子产量可达1.16＆#215;109CFU/g IDS,约是优化前的17倍.
英文摘要：
A strain, having strong antagonistic activity toward phytopathogens, was identified as Trichoderma ghanense by the methods of molecular biology. This strain of Trichoderma ghanense as testing strain, optimization of the solid state fermentation medium was performed to improve the spore yield. Through the single factor experiment, Plackett-Burman experiment, the steepest ascent experiment and the response surface analysis, sugar, corn steep liquor and MgSO4were identified as the most important factors to influence the spore yield. The optimal concentration of the three factors is 0.56% , 0.56% and 2.59% respectively. The optimal initial water content of the medium was identified as 35% and the optimal initial pHwas identified as 9. The highest spore yield have been achieved as 1.16 × 10^9 CFU/g initial dry substrate （IDS） on the optimized solid state medium, which is 17 times higher than the initial medium.
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