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中文摘要：
从土壤中筛选获得高产（＋）γ-内酰胺酶的微生物菌株,并鉴定和保藏为Delftia sp. CGMCC No. 5755。对该Delftia sp菌株的发酵产酶条件进行了研究,结果表明,最适发酵培养基为：蔗糖30 g/L,蛋白胨30 g/L,牛肉膏25 g/L,乙酰胺5 g/L,MgSO4 1 g/L;最适发酵温度及初始pH分别为32℃和pH 7.0。该菌株在上述条件下发酵培养20 h,菌体生物量为16.0 g/L,（＋）γ-内酰胺酶的酶活为692 U/L。采用Delftia sp.静息细胞对100 g/L的外消旋底物2-氮杂二环--庚烷-5-烯-3-酮（简称（±）γ-内酰胺）的水解拆分反应中,产物（-）γ-内酰胺光学纯度大于99.9%e.e.,转化率为53.7%。研究为生物催化法高效制备光学纯（＋）γ-内酰胺提供了可行的途径。
英文摘要：
A （＋）γ-lactamase-producing microorganisms with high enantioselectivity was isolated from soil, and was identified and deposited as Delftia sp.CGMCC No.5755. The optimized fermentation medium consists of： 30 g/L sucrose, 30 g/L peptone, 25 g/L beef extract, 5 g/L acetamide, and 1 g/L MgSO4; and the optimum temperature and initial pH are 32℃ and pH 7.0, respectively. Under the optimized conditions, 15.6 g/L of Delftia cells and 687 U/L of （＋）γ-lactamase were obtained after 20 h of fermentation. Biotransformation using Delftia resting cells was carried out at 100 g/L of （±）γ-lactam substrate, the optical purify of product （-）γ-lactam reached over 99.9%e.e., and the conversion rate was 53.7% after 12h.The study provides a feasible approach for the biocatalytic preparation of （-）γ-lactam.
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