中文摘要：

目的动态观察多种营养素和蛋白质协同拮抗氟中毒对大鼠卵巢损伤的效果。方法将120只断乳2周清洁级SD雌性大鼠按体重随机分为4组,分别为对照组、氟中毒组、营养素干预组、营养素＋强化蛋白干预组,每组30只。对照组大鼠食用非病区玉米加工饲料（含氟5.2 mg/kg,蛋白含量为18%）;氟中毒组大鼠食用病区高氟泥煤烘烤玉米加工饲料（含氟96 mg/kg,蛋白含量为18%）;营养素干预组大鼠饲料是在氟中毒组饲料基础上加入多维营养素添加片（含氟96 mg/kg,添加片剂量为2.08 g/kg,蛋白含量为18%）;营养素＋强化蛋白干预组大鼠饲料在营养素干预组饲料的基础上加入大豆蛋白粉（含氟96 mg/kg,添加片剂量为2.08 g/kg,蛋白含量为30%）。采用自由摄食的方法进行染毒。分别于第60、120、180天,检测处于动情期大鼠血清中睾酮（T）、雌二醇、孕酮（P）、抑制素（Inh）的含量。结果氟中毒组大鼠血清P、雌二醇、Inh水平降低,而T水平升高。两干预组血清P、雌二醇、Inh、T的紊乱程度明显减轻,且随着时间的延长逐渐恢复正常水平。第180天时两干预组血清P、雌二醇水平已经恢复到正常水平;而血清T、Inh水平介于染氟和对照组之间,且更接近对照组。结论多种营养素对氟中毒所致大鼠血清卵巢相关激素的分泌紊乱具有拮抗作用,加入蛋白质后对损伤的拮抗作用有所增强。

英文摘要：

Objective To dynamically observe the combined effects of compound nutrients and protein on damage of ovaries of rats caused by fluorosis. Methods A total of 120 healthy SD female rats were randomly divided into control group,fluoride group,compound nutrients group,compound nutrients plus enriched protein group,30 rats in each group. The rats in the control group were fed with corn baked by the materials from non-disease area（fluoride content was 5.2 mg/kg,protein content was 18%）,fluoride group were fed with corn baked by high fluoride coal from disease area（fluoride content was 96 mg/kg, protein content was 18%）,compound nutrients group,compound nutrients was added in additional to that of the fluoride group（fluoride content was 96 mg/kg,compound nutrients was 2.08 g/kg,protein content was 18%）,and the last group,with materials enriched protein based compound nutrients group（fluoride content was 96 mg/kg,compound nutrients was 2.08 g/kg,protein content was 30%）. The treatment for all rats was conducted through food. After 60,120 and 180 days of treatment respectively,serum concentration of testosterone（T）,estradiol（E2）,progesterone（P）,inhibin（Inh） were determined during the estrous period. Results Compared with the control group,serum T level in fluoride group was increased,while E2,P,Inh were decreased. Compared with the fluoride group,disorders of serum E2,P,T,Inh in the two intervention group significantly reduced and the concentration gradually return to normal level as the time extended,moreover,at 180 th day,serum E2 and P in the two intervention groups return to normal level,serum T and Inh level were between the fluoride group and control group,and much closer to the control group. Conclusion In the present study,compound nutrients display an obvious antagonistic effect on ovary-related hormones secretion disorder and the influences is more obviously at the presence of enriched protein.