中文摘要：

目的观察燃煤污染型氟中毒对雌鼠下丘脑-垂体-卵巢性腺轴系统内分泌的影响，并探索其可能机制。方法选用60只SD雌鼠，采用随机数字表法，按体质量分为2组：对照组、染氟组，每组30只。对照组食用非病区玉米饲料，染氟组自由食用贵州省织金县燃煤污染型氟中毒病区原煤烘烤的玉米饲料，以复制氟中毒动物模型。观察各组雌鼠染氟期间牙齿变化情况。喂养180d后，于动情期收集24h尿液，采用氟离子选择电极法测定尿氟含量；动情期处死雌鼠，取右侧长骨采用高温灰化．氟离子选择电极法测定骨氟含量。采用股动脉取血法收集雌鼠血液，分离血清，电化学发光放射免疫法检测血清中卵泡刺激素（FSH）、黄体生成素（LH）、睾酮（T）、雌二醇（E2）、孕酮（P），酶联免疫吸附试验（ELISA）法检测血清中促性腺释放激素（GnRH）、抑制素（INH）含量。分离下丘脑、垂体、卵巢并称重，计算下丘脑、垂体、卵巢的脏器系数；制备组织切片，光镜下观察下丘脑、垂体、卵巢组织形态；电镜观察卵巢组织亚微结构。结果成功复制实验动物模型。染氟组雌鼠下丘脑、垂体、卵巢脏器系数（0．032±0．004、0．014±0．008、0．037±0．009）与对照组（0．035±0．005、0．012±0．006、0．035±0．004）比较，差异均无统计学意义（f=0．46、0．87、0．64，P均〉0．05）。染氟组雌鼠血清GnRH、FSH、LH、T含量[（21．654±4．765）、（29．580±5．221）、（53．988±6．506）、（23．962±2．255）μg／L均高于对照组[（10．384±2．250）、（19．217±4．743）、（30．314±4．443）、（7．883±1．973）μg／L，t=6．762、4．646、9．503、16．971，P均〈0．05]；而P、INH含量[（12．635±3．841）、（18．926±3．465）μg／L]均低于对照组[（21．045±4．768）、（48．076±3．525）μg／L，t=4．344、18．649，P均〈

英文摘要：

Objective To observe the influence of coal-burning type of fluorosis on hypothalamic-pituitary- ovary axis function and to explore possible mechanism in female rats. Methods Sixty SD rats were divided into two groups according to body weight with the method of random number table： control group and fluorosis group, 30 rats in each group. Fluorosis group was feed with corn powder baked by high fluorine coal from Zhijin area. Changes of female rats＇ teeth during fluorine exposure were observed. After feeding for 180 days, 24 h urine was collected in estrus and fluorine level was tested using fluoride ion-selective electrode; rats were executed and bone fluorine levelwas tested with high-temperature ashing-fiuorine ion-selective electrode. Femoral artery blood was collected and serum was separated to test the contents of follicle stimulating hormone （FSH）, luteinizing hormone （LH）, testosterone （T）, estradiol （E2） and progesterone （P） with electrochemiluminescence radioimmunoassay and gonadotropin-releasing hormone （GnRH）, inhibin （INH） with enzyme-linked immunosorbent assay （ELISA）, respectively. Organs, including hypothalamus, pituitary gland and ovary were weighted, and organ coefficients were calculated. Pathological morphology of hypothalamus, pituitary gland and ovary was observed after staining and uhrastructure of ovary was examined by electron microscopy. Results Coal-burning induced fluorine poisoning rat model was established successfully. There were no significant differences statistically in organ coefficients between fluorosis groups （0.032 ± 0.004, 0.014 ± 0.008, 0.037 ± 0.009） and controls （0.035 ± 0.005, 0.012 ± 0.006, 0.035 ± 0.004, t = 0.46, 0.87, 0.64, all P 〉 0.05）. Rats serum GuRH, FSH, LH and T levels [（21.654 ± 4.765）, （29.580 ± 5.221）, （53.988 ± 6.506）, （23.962 ± 2.255）μg/L] of fluorosis groups were all higher than those of controls [（10.384 ± 2.250）, （19.217 ± 4.743）, （30.314 ± 4.443）, （7.883 ± 1.973?