中文摘要：

目的：观察淫羊藿苷对SAMP8小鼠记忆能力、神经元微观形态及GSK-3β蛋白表达的影响,为临床治疗提供依据。方法：采用随机、平行对照设计将所选动物分为4组,正常组、模型组、安理申组、淫羊藿苷组,每组各10只,淫羊藿苷组的小鼠灌胃剂量为20mg/kg,安理申组的小鼠灌胃剂量为1.3mg/kg,药液浓度均为2mg/mL。模型组予蒸馏水灌胃10mL/kg,时程长为4周,灌胃结束后进行水迷宫,电镜,Western检测。结果：（1）模型组与正常组比较在水迷宫实验中逃避潜伏时间明显增长,安理申组与淫羊藿苷组可以改善这一现象。在第一天的训练中淫羊藿苷组、模型组和正常组没有表现出明显统计学意义（P〉0.05）。在随后的四天中淫羊藿苷组和安理申组的小鼠潜伏期明显低于模型组,均有明显统计学意义（P〈0.05）。在第五天的在空间探索实验中淫羊藿苷组、安理申组穿越平台次数明显多于模型组,其中淫羊藿苷组最为明显。（2）电镜下观察发现,模型组与正常组比较神经元细胞器丰富度明显降低,各个细胞器结构有明显损害,淫羊藿苷组与安理申组可以保护细胞器的完整程度,降低细胞水肿程度,使线粒体,内质网等细胞器相对完整,其中淫羊藿苷组在降低细胞水肿方面优于安理申组,保护线粒体以及内质网方面相差不大。（3）Western检测发现海马DG区模型组GSK-3β表达量升高最明显,淫羊藿苷组与安理申组表达量相对较少,安理申组相对更明显,两组均介于模型组与正常组之间。结论：淫羊藿苷可改善模型小鼠学习记忆能力,该作用可能与保护小鼠海马神经元结构,降低神经纤维缠结相关蛋白表达相关。

英文摘要：

Objective ： The aim was to observe the effects of memory, neuronal morphology and GSK - β expression to SAMP8 mice with icariia and to provide basis for clinical treatment. Methods ： It was a randomized and parallel group study and we divided the mice into 4 groups：normal group, model group, aricept group and icariin group with 10 mice in each group. The dosage of 20 mg/kg was administered icariin group by gavage and the dosage of I. 3 mg/kg was administered aricept group by gavage ,2 mg/ml medicine fluid density of both groups. The model group was administered with distilled water intragrastic infusion. After 4 weeks preparation, we had Morris water maze test, observed by electron microscope, and had Western blot test. Results ： （ 1 ） In the Morris water maze test, the latent time was obviously prolonged in model group and the normal group while the phenomenon had become better in aricept group and icraiin group. In the first training, there was no statistical significance （ P 〉 0.05 ） in icariin group, model group and normal group group. The next four days, the latent time of icariin group and aricept group was shorter than the one of model group with clearly statistical significance （ P 〈 0.05 ）. On the fifth day, the number of crossing the target quadrant was evidently more in icariin group and aricept group than in model group, and the icariin group was most evident. （2） Under electron microscope,we observed that the abundance of neuronal cells was visibly decreased and each cell structure was with obvious damage in model group, whereas the organelles integrity was preserved especially mitochondria and endoplasmic reticulum, and the cell edema was relieved,and icariin group was better than aricept group in reducing the cell edema, Little difference in protecting the mitochondria and endoplasmic reticulum. （3）By Western Blot,GSK -3β expression in hippocampal dentate gyros was perceptibly more in model than in icariin group and aricept group. That of aricept group was more obv