中文摘要：

离筛选低温环境中T-2毒素的降解菌并探明其生化特性,探究T-2毒素降解微生物生化特性异同点,为T-2毒素降解微生物的检验提供生化判断参考。针对暴露于-20℃低温环境中的低浓度T-2毒素标准品,采用LC-MS/MS定量分析T-2毒素残留量,利用营养琼脂培养基（NA）和马铃薯葡萄糖琼脂培养基（PDA）共分离出5株降解菌,16S rDNA结合生化鉴定结果为死谷芽胞杆菌（Bacillus vallismortis）、蜡状芽胞杆菌（Bacillus cereus）、阴沟肠杆菌（Enterobacter cloacae）、弯曲假单胞菌（Pseudomonas geniculata）和尼泊尔葡萄球菌（Staphylococcus nepalensis）。这些分离株在-20℃条件下对低浓度T-2毒素均有不同程度的降解能力,其中蜡状芽胞杆菌（Bacillus cereus）降解能力最强,降解率为91%。它们的理化特性具有很多相似性,主要表现在5株菌均不能利用D-塔格糖（d TAG）,对ELLMAN都表现出阴性;Bacillus vallismortis Bp1234-7、Pseudomonas geniculata Bp24-4、Staphylococcus nepalensis Bp124-5和Enterobacter cloacae Bp123-7都能分解D-葡萄糖,而Bacillus cereus Bp1234-8不能。

英文摘要：

Abstract T-2 toxin degradable bacteria （T＇FDB） in low-temperature environment was Isolated and screened and found out its biochemical characteristics, and probed deeply into its microbiological and biochemical features of simi- lar and dissimilar points, to provide reference for biochemical judgment during the inspection with TrDB. Aiming at exposure of low concentration T-2 toxin standard sample to - 20 ~C low temperature environment T-2 standard toxin of low concentration, LC-MS/MS was adopted to quantitatively analyze the residual amount of T-2 toxin, using NA and PDA media, and isolated five degradable bacteria, they were identified as Bacillus vallismortis, Pseudomonus ~eniculata. Staphylococcus nepalensis. Bacillus cereus, and Enterobacter cloacae accordin~ to 16S rDNA and their biochemical characteristics, all can degrate low concentration T-2 toxin in different degrees at - 20 ~C , among them B. cereus had the strongest degradation with the rate as high as 91%. They have many similarities in physicochemical property, all of them cannot utilize D-tagatose （dTAG）, exhibited negative to ELLMAN. B. vallismortis Bp1234-7, P. geniculata Bp24-4, S. nepalensis Bp124-5 and E. cloacae Bp123-7 decompose D-glucose, while B. cereus Bp123-7 does not.