为了探讨对虾肌肉氨基酸含量与饲料中T-2毒素暴露剂量的相关性,利用不同剂量T-2毒素的染毒饲料喂养凡纳滨对虾,养殖20d后检测各试验组对虾肌肉的17种氨基酸含量,并作氨基酸评分和灰色关联分析,从而获得对虾肌肉氨基酸含量的差异程度和相关程度。氨基酸评分结果显示,当T-2毒素暴露剂量≤1.2mg/kg饲料时,对虾肌肉必需氨基酸指数升高,当T-2毒素剂量〉1.2mg/kg饲料时,必需氨基酸指数则降低;灰色关联结果显示,17种氨基酸含量的变化与T-2毒素剂量均具有显著相关性,而且每种氨基酸的关联度非常接近,其中蛋氨酸与T-2毒素剂量具有最大关联。结果表明,T-2毒素对对虾肌肉氨基酸具有低剂量刺激和高剂量抑制的特点,其中蛋氨酸受抑制影响最大。
In order to study the amino acids contents of muscle in Penaeus vanmamei exposed to T-2 toxin, feeds contaminated by different dosages of T-2 toxin were fed to different groups of Po vanmamei, and after 20 days, the contents of 17 kinds of amimo acids of P. vanmamei in different groups were detected, and then amino acid score was calculated and grey relational analysis was conducted. The results of amino acid score showed that, when T-2 toxin content was 1.2 mg/kg feed or less, the essential amino-acid total score increased, and when T-2 toxin content was above 1.2 mg/kg feed, it reduced. The results of grey correlation analysis showed that there were significant correlations between 17 kinds of amino acids and T-2 toxin content, and the correlations of 17 kinds of amino acids were very close, Methionine had the largest correlation with T-2 toxin content. The results indicated that low T-2 toxin content had stimulation on amino acids of prawn muscle, while high T-2 toxin content had inhibition, and the inhibition effect on Methionine was the most.