目的:优化云南松松塔中原儿茶酸的提取工艺。方法:以不同溶剂对云南松松塔中原儿茶酸的提取效率为指标,用高效液相色谱法进行含量测定,采用L9(3^4)正交设计试验以确定最佳提取工艺。结果:最佳提取工艺:以乙酸乙酯为提取溶剂,加入0.1 mol/L的HCl 3 mL,料液比为1∶40(mg/mL),超声时间90 min,超声温度40℃。结论:优化后的提取工艺简单、稳定、提取率高。
Objective:To optimize the extraction process of the pinecone of protocatechuic acid in Pinus yunnanensis. Methods: Different solvents were used to extract protocatechuic acid in Pinus yunnanensis. Their extraction efficiency was taking as index; content was determined by using HPLC chromatography. Finally,L9(3^4)orthogonal design experiment was used to determine the optimum the extraction process. Results:The optimum extraction process:with ethyl acetate as the extraction solvent,0.1 mol/L HCl 3 mL was added into it. Material-liquid ratio was 1∶40(mg/mL);ultrasonic time 90 min;ultrasonic temperature 40 ℃. Conclusion: The Optimized extraction process is simple,stable and have high extraction rate.