中文摘要：

建立了高效液相色谱法分析并测定华山松松塔多糖中的单糖。以三氟乙酸水解华山松松塔多糖，水解物加入1-苯基-3-甲基-5-吡唑酮（PMP）进行衍生化。使用AgilentVenusilXBP—C18色谱柱，以乙腈．0．05mol／L乙酸铵缓冲液（pH5．5）（20：80）为流动相，检测波长250nln。华山松松塔多糖由10种单糖（其中3种未鉴定）组成。已鉴定出的7种单糖均在0．005～0．2mg／ml范围内线性关系良好，回收率为89．2％～101．6％，RSD〈3．0％。在华山松松塔提取物C、D、E和F中的D-甘露糖、鼠李糖、D-葡萄糖醛酸、D-半乳糖醛酸、葡萄糖、半乳糖和D．阿拉伯糖的相对平均含量分别为0．144％～1．24％、0．13％～2．14％、0～0．99％、0．17％～2．27％、0．76％～2．73％、0．14％～9．17％和0．16％～8．16％。

英文摘要：

An HPLC method was established for the analysis and determination of the monosaccharides in polysaccharides from pinecone of Pinus armandi Franch.. The sample was hydrolyzed by trifluoroacetic acid and the hydrolysate was derivatized by reaction with 1-phenyl-3-methyl-5-pyrazolone （PMP）. An Agilent Venusil XBP-C18 column was used with the mobile phase of acetonitrile-0.05 mol/L ammonium acetate buffer （pH 5.5） （20 ： 80） at the detection wavelength of 250 nm. The polysaccharides were composed of ten monosaccharides （including three unidentified monosaccharides）. The calibration curves for the seven identified monosaccharides were linear in the range of 0.005 - 0.2 mg/ml. Their recoveries were 89.2% - 101.6%, with RSDs less than 3%. In extractions C, D, E and F from pinecone of Pinus armandi Franch., the relative average contents of mannose, rhamnose, D-glucuronic acid, D-（＋） galacturonic acid, glucose, glactose and D-arabinose were 0.144 % - 1.24 %, 0.13 % - 2.14 %, 0 - 0.99 %, 0.17 % - 2.27 %, 0.76% - 2.73%, 0.14% - 9.17% and 0.16% - 8.16%, respectively.