建立了高效液相色谱法测定云南松松塔中脱氢枞酸的含量,采用PhenomenexGemini—NXC18 110A型色谱柱,以乙腈-0.3%磷酸(58:42)为流动相,检测波长214nm。并考察不同采收时间云南松松塔中脱氢枞酸含量的动态变化。结果显示,不同采收季节云南松松塔中脱氢枞酸的含量有显著差异,5月含量最高。
An HPLC method was established for the determination of dehydroabietic acid in pine cones of Pinus yunnanensis Franch. A Phenomenex Gemini-NX C18 column was used with the mobile phase of acetonitrile-0.3 % phosphoric acid (58 : 42) at the detection wavelength of 214 nm. The results showed that the content of dehydroabietic acid from pine cones of Pinus yunnanensis Franch was varied with different collection time and the highest appeared in May.