中文摘要：

目的：研究自噬在顺铂致宫颈癌Hela细胞死亡中的作用,以及自噬基因Beclin 1对Hela细胞顺铂敏感性的影响.方法：不同浓度顺铂作用Hela细胞后,丹磺酰戊二胺（MDC）法观察细胞中自噬体的形成,蛋白质印迹法（Western blot）检测自噬基因Beclin 1和LC3蛋白的表达;将自噬基因Beclin 1的真核表达载体pcDNA3.1（＋）-Beclin1转染Hela细胞,Western blot检测Beclin 1在Hela细胞中的蛋白表达,四甲基偶氮唑蓝（MTT）比色法检测顺铂对Hela半数抑制浓度（IC50）的变化,流式细胞仪检测自噬细胞与凋亡细胞的百分率.结果：顺铂作用Hela细胞后,细胞内自噬体形成增加,自噬蛋白Beclin 1和LC3表达增加,且均呈现浓度依赖性（P＜0.05）;pcDNA3.1（＋）-Beclin 1转染Hela细胞后,Beclin 1蛋白表达增加（P＜0.05）,MTT检测IC50由转染前的1.0 mg/L下降到0.5 mg/L,凋亡细胞百分率和自噬细胞百分率均升高,与对照组相比差异有统计学意义（P＜0.05）.结论：自噬参与了顺铂对宫颈癌Hela细胞的细胞毒性作用,自噬基因Beclin 1过表达能够增加Hela细胞对顺铂的敏感性.

英文摘要：

Objective：To investigate the effects of autophagy on cisplatin-induced cervical cancer Hela cell death,and observe impact of Beclin 1 on sensitivity of Hela ceils to cisplatin in vitro. Methods： Hela cells were treated with cisplatin in different concentration, and MDC dyeing was explored to measure the formation of autophagic vacuoles and Western blot was used for detecting protein expression of Beclin 1 and LC3. The eukaryotic expression vector of Beclin 1 was transfected via lipofectamine into Hela cells. MTT assay was employed to observe the effect on the ICs0 value of cisplatin, and flow cytometry was employed to observe the percentage of autophagic ceils and apoptotic cells. Results：The autophagy vesicles were increased and the expression of Beclin 1 and LC3 were significantly improved in a dose-dependent manner in Hela ceils treated with cisplatin （P〈 0.05 ）. The eukaryotic expression vector pcDNA3.1 （＋）-Beclinl significantly improved the expression of Beclinl in Hela cells （P〈 0.05）. MTY assay showed the IC50 value of cisplatin decreased from 1.0 mg/L to 0.5 mg/L（P〈 0.05）, and more autophagic cells and apoptotic cells were identified in pcDNA3.1 （＋）-Beclin 1 group than the other groups. Conclusions ： Autophagy play an important role in the process of cisplatin-induced Hela cells death,and Beclin 1 overexpression can increase the sensitivity of Hela cells to cisplatin.