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中文摘要：
目的观察不同参数的低频脉冲电刺激对B16-F10黑色素瘤小鼠的抑瘤效应,评估其对心脏结构及功能的影响。方法将由B16F10细胞接种产生的荷瘤BALB/c小鼠随机分为4组。治疗组选择场强20V/cm,脉宽1ms,频率分别为10Hz（10Hz组）、20Hz（20Hz组）和25Hz（25Hz组）的脉冲电刺激作用于荷瘤小鼠30min/d,以不用电刺激为对照组（每组10只）,治疗时监测各小鼠心电图变化情况。脉冲电刺激荷瘤小鼠7d后比较各组肿瘤体积及生存期的变化,HE染色观察肿瘤及心脏组织形态学变化,免疫组化法比较各组小鼠肿瘤组织中S-100B蛋白的表达变化,ELISA法检测小鼠血清肌钙蛋白T（c Tn T）及肌酸激酶同工酶（CK-MB）的水平变化。结果低频脉冲电刺激治疗15d后,10Hz组、20Hz组和25Hz组的肿瘤体积分别为463.0±33.0、248.6±34.6和229.9±15.9mm^3,显著小于对照组（2027.0±133.4mm^3,P〈0.05）,且20Hz组生存率延长率（67.4%）明显高于10Hz组（15.5%）和25Hz组（8.8%）;随着频率增加,S-100B蛋白表达依次减少;25Hz组小鼠心律失常评分及血清c Tn T、CK-MB水平显著高于对照组（P〈0.05）;10Hz组和20Hz组小鼠心律失常评分及血清c Tn T、CK-MB表达与对照组比较差异无统计学意义（P〉0.05）。结论低频脉冲电刺激对小鼠黑色素瘤具有抑瘤作用,在20V/cm、20Hz时抑制作用最强,且不影响小鼠正常的心电活动。
英文摘要：
Objective To assess the inhibitory effects of pulsed electrical stimulation at different low frequencies on B16-F10 melanoma, and evaluate its influence on the structure and function of heart in mice. Methods BALB/C mice were inoculation with melanoma cell B16F10 to reproduce melanoma. These mice were randomly divided into four groups： rats in three treatment groups received of pulsed electrical stimulation of 10, 20Hz and 25Hz respectively, with a 1-ms pulse width, field strength of 20 V/cm, and duration of 30 min/d, and no electrical stimulation was given to the control group （10 each）. ECG change in each group was recorded. Seven days later, tumor volume and survival rate were recorded. The changes in tumor and myocardial morphology were examined using HE staining. The expression of S-100B protein in tumor was assessed by immunohistochemical method. The serum levels of troponin T （cTnT） and creatine kinase isoenzyme （CK-MB） were determined by ELISA. Results After being treated with pulsed electrical stimulation for 15 days, tumor volume in 10, 20Hz and 25Hz group （463.0±33.0, 248.6±34.6, 29.9±15.9mm^3, respectively）, was significantly smaller than that in control group （2027.0±133.4mm^3, P〈0.05）. Compared with 20 Hz group （67.4%）, survival rate was significantly higher than that in groups 10Hz （15.5%） and 25Hz （8.8%）. With the increase in frequency, the positive expression of S-100B protein gradually lowered, and arrhythmia score and the serum levels of cTnT or CK-MB were significantly elevated in 25 Hz group compared with that in control group （P〈0.05）. No significant difference in the arrhythmia score or serum levels of cTnT and CK-MB was found between both 10-Hz and 20-Hz groups and control group （P〉0.05）. Conclusions Low-frequency pulsed electrical stimulation has a suppressive effect on the growth of B16F10 melanoma in mice, and the pulsed electric stimulation at 20 V/cm, 20 Hz and 1 ms are proved to be the most effective in suppressing the growth of tum
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