中文摘要：

探讨了在二乙基己烯雌酚（DES）诱发成年动物生精细胞凋亡过程中睾丸一氧化氮（NO）生成和生精细胞一氧化氮合酶（eNOS和iNOs）表达的变化，以期为阐明DES诱发生精细胞凋亡机理的研究提供基础资料。成年雄性仓鼠皮下注射不同剂量DES（分别为0．01、0．1和1mg／kg体重），连续注射7d后取其睾丸，进行NO含量的测定和eNOS、iNOS免疫组化染色。电镜观察生精细胞超微结构的变化，并用TUNEL法检测睾丸中生精细胞凋亡的变化，苏丹Ⅲ染色法检测睾丸生精小管内脂滴分布的变化。结果显示：NO的生成与DES呈剂量依赖性。DES处理后，在1mg／kg体重剂量组，大量生精细胞表达eNOS和iNOS，并出现大量凋亡，退化的生精细胞胞浆内有大量髓样结构，并有大量脂滴分布于生精细胞内和细胞间。eNOS和iNOS阳性生精细胞与凋亡的生精细胞数量和类型基本一致，主要为精母细胞和圆形精子细胞。

英文摘要：

This study will explore the changes of endothelial nitric oxide synthase （eNOS） and inducible nitric oxide synthase （iNOS） expression in spermatogenic cells and the change of level of nitric oxide （NO） in adult male hamster testes during diethylstilbestrol （DES） inducing spermatogenesis apoptosis. The study will be helpful for illustrating the mechanism of DES induced adult animals spermatogenesis apoptosis. Adult male hamsters were treated daily with olive oil vehicle either alone （control） or containing various doses of DES （0. 01,0. 1 and lmg/kg body weight, respectively） （experiment） by subcutaneous injection for 7 days. The levels of NO in testes were measured. The expressions of eNOS and iNOS proteins in the testes seminiferous tubules were detected by immunohistochemistry. The changes on the histological morphology of spermatogenic cells were observed by electron microscope. The apoptotic spermatogenic cells were detected by TUNEL staining. The change of the distribution Of lipid droplets in seminiferous tubules were observed by Sudan Ⅲ staining. Results show that the level of NO was dose-dependent on DES. The apoptotic level of spermatogenic cells was similar to the level of expression eNOS and iNOS of spermatogenic cells, and these spermagenic cells were main spermatocytes and round spermatids. There are lots of marrow-like structure in degenerating spermatogenic cells cytoplasm. Lots of lipid droplets local between or in spermatogenic cells.