中文摘要：

为了探讨微量元素锌和硒互作对肉鸡肠道黏膜屏障结构的影响，将24只1日龄AA肉鸡随机分3组，分别饲喂添加有高锌高硒（锌1000mg／kg、硒5mg／kg）、低锌低硒（锌34mg／kg、硒0．08mg／kg）或常锌常硒（锌50mg／kg、硒0．15mg／kg）的日粮45d后，用免疫组化方法检测肠道内IgA^＋浆细胞及阳性反应物的数量及分布变化。结果表明；IgA^＋浆细胞及阳性物质散在分布于小肠绒毛顶端，固有层、肠腺周围以及盲肠黏膜固有层和盲肠扁桃体的淋巴小结周围。在常锌常硒组，IgA^＋浆细胞的数量及IgA阳性反应物比较多，而低锌低硒组和高锌高硒组的IgA^＋浆细胞数量及IgA阳性反应物数量减少（P〈0．01），尤其高锌高硒组减少更为明显。结论：低硒低锌或高硒高锌可抑制肉鸡肠道IgA形成，影响肠黏膜的免疫功能。日粮中按50mg／kg锌、0．15mg／kg硒的比例添加，对于维持正常肠道黏膜免疫功能是合适的。

英文摘要：

To explore the effect of trace elements zinc, selenium and their interaction on the gut barrier function in the broiler, twenty-four one-day-old AA broilers were divided into three groups, and were fed with the different diets including high zinc-selenium （Zn 1 000 mg/kg, Se 5 mg/kg）, low zinc-selenium （Zn 34 mg/kg, Se 0.08 mg/kg）, and normal zinc-selenium （Zn 50 mg/kg, Se 0.15 mg/kg）, respectively. After forty-five days, the broilers were killed and the distribution and quantity of IgA^＋-cell and IgA positive reaction substance in the gut were observed by immunohistochemistry. The results showed that the IgA^＋-cell and IgA positive reaction substance were located in the tip of villus, lamina propria, surround intestinal gland, and lymphatic nodule of cecum. In two groups of high zinc-selenium and low zinc-selenium, the quantity of IgA^＋-cell and IgA positive reaction substances were decreased（P〈0.01）, especially in the high zinc-selenium group. The distribution and the number of the IgA^＋-cell and IgA positive reaction substances were normal in the normal zinc-selenium group. This study indicated that the low zinc-selenium or high zinc-selenium would repress production of IgA, and the supplementary level of selenium at 0. 15 mg/kg and zinc at 50 mg/kg in diet should be an appropriate ratio which would keep the normal gut barrier function in the broiler.