中文摘要：

采用高效液相色谱法分析白屈菜不同器官生物碱的种类及质量比.取白屈菜不同器官粉碎,以甲醇∶水溶液（4∶1,V∶V）热回流提取,提取液蒸干后用50%甲醇溶液溶解,滤过后采用HPLC分析.采用Diamonsil C18色谱柱,流动相为乙腈-0.1%三乙胺水溶液（24∶76,三乙胺溶液用磷酸调至pH至3）,流速为1.0 m L/min,柱温为30℃,检测波长为290 nm.白屈菜碱、白屈菜红碱、血根碱、小檗碱、黄连碱分离良好,各成分质量浓度与峰面积在测定范围内均呈良好的线性关系（r〉0.999 6）,重现性良好,5种成分的RSD值（n=5）均小于2.0%;平均加样回收率（n=5）为97.78%~99.63%.白屈菜不同器官所含生物碱的种类有所差异,根、茎及果实中所含生物碱的种类较多,质量比较高.明确了白屈菜碱各器官所含主要生物碱种类,为白屈菜的合理开发和利用奠定了基础;所建立的白屈菜多种生物碱同时测定的HPLC方法稳定可靠,简便易行,可用于白屈菜药材及其制剂的质量评价.

英文摘要：

To analyze the types and contents of alkaloids in different organs of Chelidonium majus L. by HPLC, different organs of Chelidonium majus L. were smashed and extracted by heat reflux extraction method with methanol： water solution （4： 1, V： V）. The extracting solution was dried by distillation and dissolved by 50% methanol. After filtration, the sample solution was analyzed by HPLC. Diamonsil C18 reverse phase column was used as chromatographic analysis column. The mobile phase was considered as acetonitrile -0.1% triethylamine （24： 76, pH value was adjusted to 3 by phosphoric acid）. The flow rate was 1 mL/ min, column temperature was 35 ℃ and the UV detection wave length was 290 nm. Results showed chelerythrine, sanguinarine, berberine and coptisine were well separated. The mass concentrations and peak areas of different constituents showed a good linear relationship in the range of measurement （ r 〉 0. 999 6）. The experiment also showed an excellent repro-ducibility and the RSD （ n = 5 ） of 5 alkaloids were all less than 2.0%. The average sample adding recovery rates （ n = 5 ） were 97.78% - 99.63%. There were some notable differences of the types of alkaloids in different organs of Chelidonium majus L. The types of alkaloids in roots, stems and fruits were more varied, and the contents were also higher. The types of alkaloids in different organs of Chelidonium majus L. were clarified which laid a foundation for the reasonable development and utilization of Chelidonium majus L.. The developed HPLC determination method was stable, reliable, simple and convenient, which could be used for the quality evaluation of medicinal materials and preparations of Chelidonium majus L.