中文摘要：

目的探讨RASSF1A和p16 mRNA在非小细胞肺癌（NSCLC）组织中表达的意义。方法采用RT—PCR检测96例NSCLC患者癌组织及相应的远癌正常肺组织中RASSF1A和p16 mRNA。结果①RASSF1A和p16 mRNA在远癌正常肺组织中均正常表达;在NSCLC组织中，RASSF1A mRNA表达下调或缺失率达53．12％（51／96）．P16 mRNA达36．46％（35／96）。②RASSF1A mRNA下调或缺失与NSCLC病理分期和淋巴结转移有关。⑧p16 mRNA表达下调或缺失率女性（52．38％）高于男性（24．07％），50岁以上组（44．93％）高于50岁以下组（14．81％），腺癌（48．15％）高于鳞癌（21．43％），Ⅲ～Ⅳ期NSCLC下调或缺失率（45．61％）高于Ⅰ～Ⅱ期（23．08％），有淋巴结转移组（50．00％）高于无淋巴结转移组（13．89％），P均＜0．05。④NSCLC组织中RASSF1A和p16 mRNA表达呈正相关（ra=0．278，P＜0．05）。结论RASSF1A和p16 mRNA在NSCLC中表达下调或缺失率较高，二者可能参与调控NSCLC的发生和发展。

英文摘要：

[Objective] To explore the trans-crlptional expression of Ras association domain family 1A gene （RASSF1A） and p16 mRNA in non-small cell lung cancer （NSCLC） tissues. [Methods] RT-PCR was used to detect the expression of RASSF1A and p16 gene mRNA in 96 human NSCLC tissues and matched 96 normal lung tissues far from cancer area. [Results] ①The transcriptional expression of RASSF1A and p16 gene was normal in all normal lung tissues far from cancer area but lower or loss in 53.12%（51/96） carcinoma tissues for RASSF1A and in 36.46% （35/96） carcinoma tissues for p16 gene. ②The rate of low or loss of RASSFIA mRNA was significantly higher in the patients in advanced stages （61.04 % ） and in the patients with positive lymph node metastasis （65%） than in those in early stage （41.03%, P 〈 0.05） and in those without lymph node metastasis （33.33%, P 〈 0. 05）. ③The rate of low or loss of p16 gene mRNA was remarkably higher in female group （ 52.38 % ）, more than fifty years group （44.93 % ）, adenocarcinoma group （48. 15 % ）, stage Ⅲ and Ⅳ tumors （45.61%） and lymph node metastasis group （50.00%） than that in male group（24.07%）,less than fifty years group （14.81%）,squamous cell carcinoma group （21.83%）,stage Ⅰ and Ⅱ tumors（23.08%） and non-lymph node metastasis group （13.89）, P 〈 0. 05. ④The positive correlation between the transcriptional expression of RASSF1A and that of p16 gene was identified in 96 NSCLC tissues. [Conclusion] Loss or abnormal downregulation of RASSF1A and p16 mRNA is a frequent event in patients with NSCLS in Chlnese,which may play an important role in the malignant progress and prognosis of NSCLS.