中文摘要：

目的：探讨非小细胞肺癌（NSCLC）组织中磷酸化AKT（p-AKT）和细胞增殖核抗原（PCNA）的表达。方法：应用免疫组化法检测80例NSCLC组织和35例非肿瘤性肺组织标本中p-AKT蛋白和PCNA的表达,分析p-AKT蛋白表达与NSCLC患者临床病理因素的关系。结果：非肿瘤性肺组织中p-AKT蛋白不表达,在NSCLC组织中的阳性表达率为78.8%（63/80）,2组比较差异有统计学意义（P〈0.05）;p-AKT蛋白的表达与患者年龄、性别、组织学类型及鳞癌的分化程度、TNM分期、淋巴结转移无关（P〉0.05）,仅在高中分化腺癌组织中的阳性表达率（95.0%（19/20））高于低分化腺癌组织中的阳性表达率（50.0%（8/16）,P〈0.05）。在NSCLC和非肿瘤性肺组织中,PCNA标记指数分别为（52.45±26.14）%和（9.57±2.20）%,2者相比差异有统计学意义（P〈0.05）。NSCLC组织中p-AKT蛋白的表达与PCNA呈正相关（r=0.376,P〈0.05）。结论：在NSCLC中存在AKT的活化,p-AKT的表达与肿瘤的高增殖活性有关。

英文摘要：

Aim： To investigate the expression of phosphorylated AKT（p-AKT） and proliferating cell nuclear antigen （PCNA） in human non-small cell lung cancer （NSCLC） tissue and their correlations. Methods：The expression of p-AKT and PCNA in 80 cases of NSCLC and 35 cases of non-cancerous lung tissue were assessed by immunohistoehemistry, and their correlations with eliniopathological factors were analyzed. Results： The positive rate of p-AKT in lung cancer tissue （ 78.8 % ） was significantly higher than that of p-AKT in non-cancerous lung tissue （0.0%） （ P 〈 0. 05 ） ; the expression of p-AKT did not relate to age, sex, histologie subtype, squamous cancer differentiation, lymph node metastasis or TNM stages（P 〉0.05 ）. The positive rate of p-AKT in well-differentiated and moderately-differentiated adenoeareinoma tissue （95.0%） was significantly higher than that in poorly-differentiated adenoeareinoma tissue （50.0%） , P 〈 0. 05. PCNA labeling indexes in NSCLC and non-cancerous lung tissue were （52.45 ± 26.14） % and （9.57 ± 2.20） % , P 〈 0. 05. In NSCLC tissue, the expression of p-AKT was positively correlated with PCNA （ r = 0. 376, P 〈 0. 05 ）. Conclusion ： AKT activation may be present in NSCLC. The expression of p-AKT is related to the high proliferating activity of tumor cells.