中文摘要：

目的筛选并鉴定存在明显表达差异的蛋白质，为人肺腺癌发病机制及其早期诊断的研究提供理论依据。方法采用固相pH梯度双向凝胶电泳技术分离12例Ⅰ期肺腺癌及其相配的癌旁正常肺组织可溶性总蛋白，建立人Ⅰ期肺腺癌及相配癌旁正常肺组织蛋白质的双向电泳凝胶图谱；PDQuest凝胶图像分析软件比较分析，筛选出差异表达的蛋白质点；基质辅助激光解吸电离飞行时间质谱获得相应蛋白质点的肽质量指纹图谱；搜索蛋白质数据库鉴定差异表达的蛋白质。结果建立了重复性较好的人Ⅰ期肺腺癌及相配癌旁正常肺组织蛋白质的双向电泳凝胶图谱；筛选出存在明显表达差异的蛋白质点26个，挖取其中的9个蛋白质点进行质谱分析，9个蛋白质点均得到了满意的肽质量指纹图谱；搜索蛋白质数据库鉴定出4种蛋白质，分别是：608核糖体蛋白P2、组织蛋白酶B1、载脂蛋白A—Ⅰ前体和La4．1蛋白。结论成功建立了人Ⅰ期肺腺癌及相配癌旁正常肺组织蛋白质的双向电泳凝胶图谱，鉴定出4种在肺腺癌发生早期出现明显表达变化的蛋白质，为进一步探索人肺腺癌的发病机制及寻找特异性的早期分子标志物提供了理论依据。

英文摘要：

Objective To screen and identify differential expression proteins, and to obtain theoretical data for studying human stage Ⅰ lung adenocarcinoma molecular mechanism and finding early biomarker. Methods The total proteins of 12 human stage Ⅰlung adenocarcinoma tissue and normal tumor-adjacent tissue were separated, using 170 mm long immobilized pH gradient （IPG） gel strips, pH 3-10, for the first dimension and homogeneous SDS-polyacrylamide gel electrophoresis （SDS-PAGE）（12%T） for the second dimension. The differential expression proteins were analyzed with PDQuest image analysis software, then identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry （MAL- DI-TOF-MS） and database searching. Results The well-reproducible 2-DE gel patterns of human stage Ⅰ lung adenocarcinoma and normal tumor-adjacent tissue were established and 26 differential proteins were screened. Nine of 26 differential protein spots were cut out from the preparation gels and were identified with MALDI-TOF-MS. Comparing with the protein database, four candidate proteins were identified with MS. They were 60S acidic ribosomal protein P2,Cathepsin B precursor, Apolipoprotein A-Ⅰ precursor and La 4. 1 protein. Conclusion In this study,2-DE gel images of human stage I lung adenocarcinoma and paired normal tumor-adjacent tissue were established successfully, and identified 4 differential proteins. These data will be helpful for studying human lung adenocarcinoma molecular mechanism and screening early biomarker.