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饲料蛋白质水平对大鳞鲃幼鱼消化酶活性的影响

ISSN号：1005-3832
期刊名称：《水产学杂志》
时间：0
分类：S963.73[农业科学—水产养殖;农业科学—水产科学]
作者机构：[1]中国水产科学研究院黑龙江水产研究所,哈尔滨150070, [2]东北林业大学野生动物资源学院,哈尔滨150040, [3]天津市天祥水产有限责任公司,天津301500
相关基金：基金项目：现代农业产业技术体系建设专项资金项目“北方地区大宗淡水养殖”（CARS-46）;农业部“948”引进项目“大鳞引种”（2001-475-5）;公益性行业专项（农业）项目“冷水性鱼类养殖产业化研究与示范”（201003055）;中央级科研院所科研业务费项目“冷水性鱼类投喂策略”（201103）

作者：王常安[1,2], 徐奇友[1], 徐伟[1], 许红[1], 赵志刚[1], 罗亮[1], 苗建发[3], 王洋[1]

关键词：大鳞鱼巴, 蛋白质, 消化酶, Barbus capito, protein, digestive enzyme

中文摘要：

试验研究了不同饲料蛋白质水平对初重（11．07±2．42）g大鳞纪幼鱼（Barbus capito）消化酶活性的影响。饲料蛋白质水平设7个梯度：30．24％（G1）、33．22％（G2）、36．23％（G3）、39．33％（G4）、42．15％（G5）、45．33％（G6）和48．12％（G7），每处理组设3个重复，每重复30尾鱼。试验鱼饲养周期为56d。结果表明，饲料蛋白质水平影响前肠和中肠的蛋白酶活性；G5前肠蛋白酶活性显著高于G1和G7（P〈0．05）；G4中肠蛋白酶活性显著高于G1、G6和G7（P〈0．05）。随着饲料蛋白质水平的升高，后肠蛋白酶活性先增大、后降低。各处理组前肠、中肠、后肠和肝胰腺的脂肪酶活性均无显著差异（P〉0．05）。而随着饲料蛋白质水平的升高，前肠、中肠、后肠和肝胰腺淀粉酶活性降低：G7前肠淀粉酶活性显著低于G3和G6（P〈0．05）；G7中肠淀粉酶活性显著低于G1和G4（P〈0．05）；G7后肠淀粉酶活性显著低于其它处理组（P〈0．05）；G7肝胰腺淀粉酶活性显著低于G1、G2、G3和G4（P〈0．05）。从而得出结论：饲料蛋白质水平显著影响大鳞鱼巴幼鱼的蛋白酶和淀粉酶活性，而对脂肪酶的活性影响不显著。饲料蛋白质水平为39．33％时，大鳞鱼巴幼鱼的消化酶活性最强。

英文摘要：

This trial was conducted to determine the effects of different dietary protein levels on digestive enzyme of young Barbus capito at an average weight of 11.07 2.42 g. Formulated diets contain seven protein levels of 30.24% （G1）, 33.22% （G2）, 36.23% （G3）, 39.33% （G4）, 42.15% （G5）, 45.33% （G6） and 48.12% （G7）, respectively. Each diet was randomly assigned to triplicate groups of 30 fish. Fish were raised in recycling system for 56 days. The results show that the protease activities in foregut and midgut increased with the rise of dietary protein levels. The protease activity in foregut of G5 was significantly higher than that of G1 and G7 （P〈0.05）, respectively. The protease activity in midgut of G4 was significantly higher than that of G1, G6 and G7 （P〈0.05）, respectively. The protease activity in hindgut rose up and subsequently diminished with the increase of the dietary protein levels. There were no significant differences among the groups in lipase activities of foregut, midgut, hindgut and hepatopancreases （P〉0.05）. The amylase activity in foregut of G7 was significantly higher than that of G3 and G6 （P〈0.05）, respectively. The amylase activity in midgut of G7 was significantly higher than that of G1 and G4 （P〈0.05）, respectively. The amylase activity in hepatopancreases of G7 was significantly higher than that of G1, G2, G3 and G4 （P〈0.05）, respectively. In conclusion, the dietary protein levels affected the protease and amylase activities of young Barbus capito significantly, but there were no significant changes in lipase activities. The digestive enzyme activity reached the peak when the dietary protein level was 39.33%.

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