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三种中药方剂对施氏鲟抗氧化能力的影响

ISSN号：1005-3832
期刊名称：《水产学杂志》
时间：0
分类：S942.5[农业科学—水产养殖;农业科学—水产科学]
作者机构：[1]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070
相关基金：公益性行业（农业）科研专项经费（201003055）; 中央级公益性科研院所基本科研业务费专项（201001）

关键词：中药方剂, 施氏鲟, 超氧化物歧化酶(SOD), 丙二醛(MDA), 一氧化氮(NO), 一氧化氮合酶(NOS), Chinese medicinal herb complex additive； Acipenser schrencki； SOD； MDA； NO； NOS

中文摘要：

在水温22℃下,将平均体质量为0.08±0.10 kg的施氏鲟（Acipenser schrenckii）在室内水族箱（0.90 m×0.50 m×0.45 m）中预饲两周后,每日口灌给药一次中药贯众、方剂一和方剂二水煎剂,剂量分别为9、175和36g.50kg-1体质量,口灌蒸馏水为对照组,连续给药14d。然后,取部分鱼高温30℃热刺激2h后,与常温22℃给药的实验组共同采样,测定血液和肝脏内超氧化物歧化酶（SOD）、一氧化氮合酶（NOS）的活性及丙二醛（MDA）和一氧化氮（NO）含量。结果表明,三种中药方剂均能提高施氏鲟抗氧化能力。贯众能有效提高施氏鲟组织中SOD活性,在热刺激情况下,促进鱼体快速应答,降低鱼体内MDA含量,但可能对肝脏造成一定负担,需慎重给药。方剂一能有效降低鱼体内MDA含量,促进机体对热应激做出快速有效应答反应;方剂二抗氧自由基效果较好,受高温热刺激影响不大。

英文摘要：

Effects of three Chinese medicinal herb complex additives（Cyrtomium fortunei,prescription I and prescription II） aqueous extract on the contents of active material and the activities of enzymes related to antioxidation in 1＋ age old Amur sturgeon（Acipenser schrencki Brandt） by oral perfusion.The Amur sturgeon juveniles with body weight of 0.08±0.10 kg were cultured in aquaria of each 0.90 m×0.50 m×0.45 m at water temperature of 22℃ for 2 weeks and then were orally administrated by Cyrtomium fortunei,prescription I and prescription II at a dose of 9,17.5 and 36 g·50 kg-1 body weight once a day for 14 days,respectively,oral distilled water as the control.After 14 day administration,some test fish were stimulated for two hours at high temperature（30℃）,and then all sampled together to analyze the activities of superoxide dismutase（SOD）,nitric oxide synthase（NOS） and the contents of malonaldehyde（MDA） and nitric oxide（NO）.The three prescriptions were shown to play a certain role in improvement of antioxidation in Amur sturgeon.Cyrtomium fortune additive led to increase in activity of SOD in several tissues of Amur sturgeon and to promote a rapid response of fish under the thermal stimulation.Although Cyrtomium fortune showed decrease in the content of MDA,it had a certain influence on liver antioxidation.Therefore it should be administered properly and the optimum administration modalities were required.The decrease in content of MDA was observed in the body of the fish administrated by Prescription I and improvement of a rapid response of the fish administrated by Prescription I to the thermal stimulation.Prescription II had a better performance in antioxidation,but did not show any effects on high temperature stimulation.

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