为研究维生素K3对西伯利亚鲟Acipenser baerii生长和抗氧化功能的影响,选择初始体质量为5.14 g左右的西伯利亚鲟630尾,分为7组,投喂分别添加0、5、10、20、40、80、2000 mg/kg的甲萘醌烟酰胺亚硫酸盐(MNB)饲料,测得到甲萘醌(维生素K3)实际含量为0、2.88、4.84、10.08、26.42、40.86、990.00 mg/kg(饲料),每组设3个重复,每个重复放30尾鱼,饲养试验周期为56 d。结果表明:不同水平的维生素K3对西伯利亚鲟增重率、特定生长率和饵料系数的影响都未达到显著性水平(P〉0.05),但增重率和特定生长率以26.24 mg/kg维生素K3组最高,990.00 mg/kg维生素K3组最低;与对照组相比,10.08、26.24 mg/kg维生素K3组西伯利亚鲟肝胰脏总抗氧化能力(T-AOC)显著提高(P〈0.05),990.00 mg/kg组显著低于2.88、4.84、10.08、26.24 mg/kg组(P〈0.05);4.84、10.08、26.24 mg/kg维生素K3组肾脏还原型谷胱甘肽(GSH)含量显著提高(P〈0.05),4.84、10.08、26.24、40.86 mg/kg组肾脏T-AOC能力显著提高(P〈0.05),990.00 mg/kg组肾脏GSH含量和T-AOC能力显著低于4.84、10.08、26.42、40.86 mg/kg组(P〈0.05);不同水平的维生素K3对肝胰脏和肾脏SOD及MDA的影响均未达到显著性水平(P〉0.05),对西伯利亚鲟的生长也无影响;以肝胰脏和肾脏的T-AOC为指标进行二次曲线回归分析,得到西伯利亚鲟对维生素K3的需要量分别为19.79、24.57 mg/kg(饲料)。
The present study was aimed to investigate effects of dietary vitamin K3 on growth and antioxidation in Siberian sturgeon Acipenser baerii.A total of 630 individuals of Siberian sturgeon with initial body weight of 5.14 g were randomly assigned to 7 treatments and fed diets supplemented with Menadione Nicotinamide Bisulfite(MNB)at 0,5,10,20,40,80,and 2000 m g / kg,that is,containing vitamin K3 of 0,2.88,4.84,10.08,26.42,40.86,and 990.00 mg / kg,with 3 replicates per treatment for 56 days.The results showed that levels of vitamin K3 had no significant effect(P 〉 0.05) on weight gain rate(WGR),specific growth rate(SGR) and feed conversion ratio(FCR),the maximal WGR and SGR in the 26.24 mg /kg group,and the minimal WGR and SGR in 990 mg /kg group.The total antioxidant capacity(T-AOC) in hepatopancreas was shown to be significantly higher in the fish fed the diets containing 10.08,and 26.24 vitamin K mg / kg than in the fish fed the control diet(P 〈 0.05).There was significantly lower T-AOC in 990 mg / kg group than that in 2.88,4.84,10.08,26.24 mg / kg vitamin K3groups(P 〈 0.05).The Siberian sturgeon juveniles fed the diets containing 4.84,10.08,and 26.24 mg / kg vitamin K3 had significantly higher reduced glutathione(GSH) in kidney(P 〈 0.05),and the juveniles fed the diets containing 4.84,10.08,26.42,and 40.86 mg / kg vitamin K3 had significantly higher T-AOC in kidney(P 〈0.05).However,the juveniles fed the diets containing 990.00 mg / kg vitamin K3 had significantly lower GSH and T-AOC than the juveniles fed the diets containing 4.84,10.08,26.42,and 40.86 mg / kg vitamin K3did(P 〈0.05).The levels of vitamin K3 had no significant effect on superoxide dismutase(SOD) or malondialdehyde(MDA) in hepatopancreas and kidney(P 〉 0.05).In conclusion,there was no significant effect of levels of vitamin K3 on growth in Siberian sturgeon,and quadratic regression analysis of T-AOC indicated that the optimal dietary vitamin K3 requirement was estimated to