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中文摘要：
采用赤子爱胜蚓作为受试生物，研究了土壤金霉素污染对蚯蚓生长发育的慢性毒性效应．结果表明：暴露7d时，1、10、100mg·kg-1金霉素对蚯蚓体质量均无显著影响，暴露21d后，10、100mg·kg-1金霉素对蚯蚓体质量产生显著抑制作用；1、10、100mmg·kg-1金霉素均诱导了蚯蚓体内可溶性蛋白含量的增加，且暴露浓度越高，诱导效应越强；1、10、100mg·kg-1金霉素胁迫下蚯蚓体内的超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）均有不同程度的上升；暴露28d后，蚯蚓的基因表达也产生了明显变化．表明金霉素污染对蚯蚓的生长发育具有慢性生态毒性效应，蚯蚓体质量、可溶性蛋白、抗氧化防御酶和基因表达等指标可以作为评价金霉素毒性的生物标志物。
英文摘要：
Eiseniafoetida was selected to investigate the ecotoxicological effects of chlorotetracycline on the earthworm in soil. The results showed that 1, 10 and 100 mg · kg-1 chlorotetracycline had no significant effects on earthworm＇ s body mass after a 7-d exposure, but it was significantly inhibited by 10, 100 mg · kg-1 chlorotetracycline after 21 days. The soluble protein content of earthworm was induced by 1, 10 and 100 mg · kg-1 chlorotetracycline, and showed a positive response as the concentration increased. Also,the earthworm treated by 1, 10 and 100 mg·kg-1 chlorotetracycline in- duced the increases of SOD, POD and CAT activities to different degrees. The gene expression in earthworm changed significantly after a 28-d exposure. It is suggested that chlorotetracycline had a chronic ecotoxicological effect on earthworm, and the body mass, soluble protein, antioxidant en- zyme and gene expression could be used as the biomarkers to estimate chlorotetracycline toxicity.
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