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多环芳烃芘降解菌株的培养与降解特性研究

ISSN号：1009-6094
期刊名称：《安全与环境学报》
时间：0
分类：X592[环境科学与工程—环境工程]
作者机构：[1]沈阳大学区域污染环境生态修复重点实验室,沈阳110044
相关基金：国家自然科学基金重点项目（40930739）;国家社科基金重点项目（09A2D026）;沈阳大学博士启动基金项目

关键词：环境工程学, 多环芳烃芘, 16S, RDNA, 降解特性, environmental engineering, polycyclic aromatic hydro carbons （Pyrene） , 16S rDNA, degradation characteristics

中文摘要：

采自浑河流域的底泥样品以多环芳烃芘为唯一碳源反复驯化，分离筛选出1株对多环芳烃芘具有降解作用的菌株P-D-1。经形态、生理生化特性及16S rDNA序列同源性分析，该菌株为革兰氏阳性菌。利用菌株的16S rDNA测序后的部分序列通过NCBI在线比对，与Genbank中枯草芽孢杆菌属的同源性高达99％。初步鉴定菌株为枯草芽孢杆菌属菌株（Bacillus subtilis）。该菌株的最适生长条件为30℃，pH=7．0。采用高效液相色谱（HPLC）对其降解特性及培养条件进行优化，研究不同碳源、氮源、氯化钠质量浓度和通气量对多环芳烃芘降解的影响。结果表明，芘的初始质量浓度为75mg·^-1时，在以蔗糖为碳源，酵母膏为氮源，氯化钠质量浓度为0．02g·mL^-1，在250mL三角瓶中加入100mL培养基状况下，该菌株的生长效果最佳，对多环芳烃芘的降解率达到82．6％.

英文摘要：

This paper takes it as the goal of the research to identify and characterize the PAHs （Pyrene） degrading strain and its optimal cultivation. The sediment samples used in our project come from Hunhe River, with the bacterial strain P- D - 1 cultured and isolated through long cultivation with Polycyclic Aromatic Hydrocarbon （Pyrene） as the single carbon source. To make our research successful, we have studied the Pyrene-optimized cultivation and identification of isolated P - D - 1 in this paper, which has been identified as Bacillus subtilis based on its physiological and biochemical characteristics as a result of homological analysis of 16S rDNA sequences. The P- D- 1 strain is for gram-positive organisms, partially sequenced by 16S rDNA in the strains DNA, in comparison with other Bacillus subtilis, which can be found in the NCBI Genbank online, belong to the homology of up to 99%. It was shown that the optimal cultivation conditions should be cropped up at pH 7.0 and at the temperature of 30 ℃ . The performance of the liquid chromatography （HPLC） of its degradation characteristics and cultivation condition can be made optimized with the degradation Pyrene optimized by using different carbon sources, nitrogen sources, sodium chloride concentration and the culture medium dosage. The results of our test indicate that suppose the primary concentration of the polycyclic aromatic hydrocarbon （Pyrene） was 75 mg· L^- 1, the sucrose and yeast extract were made to be the carbon resource and nitrogen source, with the concentration of sodium chloride being 0.02 g· mL^-1 in the culture medium, with 100ml culture medium in the 250ml triangle flask, under the condition of which the growth of strain P - D - 1 should be the best of all the other culture treatments, at the same time, the Pyrene degradation rate could be made to reach 82.6%.