盆栽试验研究了不同培养条件(土壤与河沙)下,3种施Cd水平(0、5、50 mg kg-1)对菌根玉米生长、根系侵染率及重金属Cd吸收的影响。结果表明:施Cd水平显著影响玉米吸Cd能力。随Cd浓度增加,各处理根系与地上部分Cd浓度和吸收量显著增加,各施Cd水平下玉米根系Cd浓度和吸收量远远高于地上部分。不同培养条件下,各处理也表现出明显的差异。以河沙为培养基质,接种菌根真菌显著增加玉米的生物量及根系侵染率;在高Cd水平下,接种处理玉米根系中的Cd浓度和吸收量较对照显著增加,但地上部分的Cd浓度和吸收量却相应降低。以土壤为培养基质,随施Cd水平提高,植株吸Cd量增加,但接种处理植株根部与地上部Cd浓度均显著低于对照。试验表明,丛枝菌根真菌Glomus mosseae可减少重金属Cd向玉米植株地上部的运输,将更多的Cd固持在菌根之中,这可能是菌根减轻宿主植物Cd毒害的一个重要机制。
In a pot experiment,the effect of inoculation with AM fungus Glomus mosseae on Cd accumulation in maize plants under different cultural condition and Cd addition levels were investigated.The results indicated that Cd addition level significantly affected Cd accumulation in plants.With increasing Cd addition level,Cd concentration and accumulation in all treatments were markedly increased,and the Cd concentration and accumulation in roots were significantly higher than those in shoots.All the treatments were also different in different cultural condition.In sand culture,inoculation increased the biomass and colonization of maize plants,and inoculated plants had higher Cd concentration and accumulation in roots but lower Cd concentration and accumulation in shoots under high Cd addition level,compared to uninoculated controls.In soil culture,although plants enhanced Cd accumulation with increasing Cd addition level,inoculated plants decreased the Cd concentration significantly in shoots and roots,as compared to control.The experiment demonstrated that Glomus mosseae could effectively reduce Cd transferring to shoots in maize plants and retain much Cd in plant roots,which might be one of the key mechanisms for the alleviation of Cd phytotoxicity by mycorrhizal associations.