采用盆栽方法,以清洁土壤为对照,研究了盐碱胁迫下不同生物有机肥施用量(0%、2%、4%和8%)对向日葵种子发芽率和出苗率、幼苗生长及生理指标的影响。结果表明:与对照相比,盐碱胁迫(T0)7~28d后,向日葵种子的发芽率及出苗率明显降低,种子出苗时间推迟了3d,幼苗株高、根长、地上部分及根系鲜重、叶片中叶绿素含量、可溶性蛋白质含量均显著降低(P〈0.05),而且幼苗根系对盐碱胁迫的敏感性明显大于地上部;与T0处理相比,生物有机肥施用量分别为2%(T1)、4%(T2)及8%(T3)时,向日葵种子的出苗率分别提高了56.4%(P〈0.05)、64.3%(P〈0.05)及85.7%(P〈0.05),出苗时间提早了2~3d,盐碱胁迫处理7d后,T1、T2和T3处理组幼苗的生长明显增强,叶片中叶绿素含量分别提高了24.4%(P〈0.05)、27.2%(P〈0.05)和33.2%(P〈0.05),可溶性蛋白质含量分别增加了34.4%(P〈0.05)、43.6%(P〈0.05)和48.8%(P〈0.05);随着处理时间的延长,T1、T2和T3处理对幼苗生长及生理指标的抑制作用均表现出不同程度的降低。综上可知,盐碱胁迫条件下,施用2%~8%的生物有机肥可以明显减轻盐碱胁迫对向日葵种子出苗、幼苗生长及生理指标的抑制作用。
A pot experiment was conducted to study the effects of different dosages(0%,2%,4%,and 8%)bioorganic fertilizer on the seed germination,emergence rate,seedling growth,and leaf physiological indices of sunflower under salt-alkali stress.As compared with the control,after 7-28 days under salt-alkali stress(T0),the seed germination rate and emergence rate decreased markedly,seed emergence delayed for 3 days,seedlings shoot-and root length,shoot-and root fresh weight,and leaf chlorophyll and soluble protein contents decreased significantly(P0.05),and the roots were much more sensitive than the shoots.As compared with T0,after the application of 2%(T1),4%(T2),and 8%(T3)bioorganic fertilizer,the seed emergence rate increased by 56.4%(P0.05),64.3%(P0.05),and 85.7%(P0.05),respectively,and the seed emergence advanced by 2-3 days.After 7 days under salt-alkali stress,the seedling growth in treatments T1,T2,and T3 had an obvious increase,leaf chlorophyll content increased by 24.4%(P0.05),27.2%(P0.05),and 33.2%(P0.05),and leaf soluble protein content increased by 34.4%(P0.05),43.6%(P0.05)and 48.8%(P0.05),respectively.When the treated duration prolonged from 7 to 28 days,the inhibitory effects of salt-alkali stress on the seedling growth and the leaf chlorophyll and soluble protein contents in treatments T1,T2,and T3 decreased to some extent.These results indicated that applying 2%-8% bioorganic fertilizer could obviously alleviate the inhibitory effects of salt-alkali stress on the seed emergence,seedling growth,and leaf physiological indices of sunflower.